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Labeling surface proteins with high specificity: Intrinsic limitations of phosphopantetheinyl transferase systems

OBJECTIVE: Fluorescent labeling of specific cell-surface proteins enables a manifold of techniques to study their function in health and disease. A frequently cited family of methods employs phosphopantetheinyl transferases (PPTases) to attach probes, provided as conjugates of Coenzyme A. This metho...

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Detalles Bibliográficos
Autores principales:	Stüber, Jakob C., Plückthun, Andreas
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2019
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6922365/ https://www.ncbi.nlm.nih.gov/pubmed/31856184 http://dx.doi.org/10.1371/journal.pone.0226579

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