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Accelerated genome engineering of Pseudomonas putida by I‐SceI―mediated recombination and CRISPR‐Cas9 counterselection

Pseudomonas species have become reliable platforms for bioproduction due to their capability to tolerate harsh conditions imposed by large‐scale bioprocesses and their remarkable resistance to diverse physicochemical stresses. The last few years have brought forth a variety of synthetic biology tool...

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Detalles Bibliográficos
Autores principales: Wirth, Nicolas T., Kozaeva, Ekaterina, Nikel, Pablo I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6922521/
https://www.ncbi.nlm.nih.gov/pubmed/30861315
http://dx.doi.org/10.1111/1751-7915.13396