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Fluoride reactivation-enabled sensitive quantification of tabun adducts on human serum albumin by GC–MS/MS via isotope dilution

Organophosphorus nerve agents inhibit the cholinesterase activity by phosphylation of the active site serine. The resulting phosphylated cholinesterase and adducts on human serum albumin (HSA) are appropriate biomarkers for nerve agents exposure. Several methods have been developed for the detection...

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Autores principales: Li, Xiao-Sen, Wu, Ji-Na, Yan, Long, Xing, Zhong-Fang, Liu, Chang-Cai, Chen, Bo, Yuan, Ling, Yang, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Newlands Press Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923783/
https://www.ncbi.nlm.nih.gov/pubmed/31729243
http://dx.doi.org/10.4155/bio-2019-0161
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author Li, Xiao-Sen
Wu, Ji-Na
Yan, Long
Xing, Zhong-Fang
Liu, Chang-Cai
Chen, Bo
Yuan, Ling
Yang, Yang
author_facet Li, Xiao-Sen
Wu, Ji-Na
Yan, Long
Xing, Zhong-Fang
Liu, Chang-Cai
Chen, Bo
Yuan, Ling
Yang, Yang
author_sort Li, Xiao-Sen
collection PubMed
description Organophosphorus nerve agents inhibit the cholinesterase activity by phosphylation of the active site serine. The resulting phosphylated cholinesterase and adducts on human serum albumin (HSA) are appropriate biomarkers for nerve agents exposure. Several methods have been developed for the detection of nerve agents, including fluoride reactivation or alkaline cleavage. It was previously thought that some nerve agents adducts to HSA could not be detected via fluoride regeneration. In our study, the results showed that tabun (GA) adducts of HSA could be detected by fluoride regeneration. The sample preparation included acetone precipitation, washing and SPE. Deuterated tabun (d(5)-GA) was applied as the internal standard. The product of regenerated fluorotabun is detected with a good linearity (R(2) > 0.997) in the concentration range from 0.02 to 100.0 ng/ml, small relative standard deviation (≤6.89%) and favorable recoveries between 94.8 and 106.3%. The established preparation confirmed the fluorotabun was regenerated from the GA-HSA adducts.
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spelling pubmed-69237832019-12-23 Fluoride reactivation-enabled sensitive quantification of tabun adducts on human serum albumin by GC–MS/MS via isotope dilution Li, Xiao-Sen Wu, Ji-Na Yan, Long Xing, Zhong-Fang Liu, Chang-Cai Chen, Bo Yuan, Ling Yang, Yang Bioanalysis Research Article Organophosphorus nerve agents inhibit the cholinesterase activity by phosphylation of the active site serine. The resulting phosphylated cholinesterase and adducts on human serum albumin (HSA) are appropriate biomarkers for nerve agents exposure. Several methods have been developed for the detection of nerve agents, including fluoride reactivation or alkaline cleavage. It was previously thought that some nerve agents adducts to HSA could not be detected via fluoride regeneration. In our study, the results showed that tabun (GA) adducts of HSA could be detected by fluoride regeneration. The sample preparation included acetone precipitation, washing and SPE. Deuterated tabun (d(5)-GA) was applied as the internal standard. The product of regenerated fluorotabun is detected with a good linearity (R(2) > 0.997) in the concentration range from 0.02 to 100.0 ng/ml, small relative standard deviation (≤6.89%) and favorable recoveries between 94.8 and 106.3%. The established preparation confirmed the fluorotabun was regenerated from the GA-HSA adducts. Newlands Press Ltd 2019-11-15 2019-12 /pmc/articles/PMC6923783/ /pubmed/31729243 http://dx.doi.org/10.4155/bio-2019-0161 Text en © 2019 Yang Yang This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Research Article
Li, Xiao-Sen
Wu, Ji-Na
Yan, Long
Xing, Zhong-Fang
Liu, Chang-Cai
Chen, Bo
Yuan, Ling
Yang, Yang
Fluoride reactivation-enabled sensitive quantification of tabun adducts on human serum albumin by GC–MS/MS via isotope dilution
title Fluoride reactivation-enabled sensitive quantification of tabun adducts on human serum albumin by GC–MS/MS via isotope dilution
title_full Fluoride reactivation-enabled sensitive quantification of tabun adducts on human serum albumin by GC–MS/MS via isotope dilution
title_fullStr Fluoride reactivation-enabled sensitive quantification of tabun adducts on human serum albumin by GC–MS/MS via isotope dilution
title_full_unstemmed Fluoride reactivation-enabled sensitive quantification of tabun adducts on human serum albumin by GC–MS/MS via isotope dilution
title_short Fluoride reactivation-enabled sensitive quantification of tabun adducts on human serum albumin by GC–MS/MS via isotope dilution
title_sort fluoride reactivation-enabled sensitive quantification of tabun adducts on human serum albumin by gc–ms/ms via isotope dilution
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923783/
https://www.ncbi.nlm.nih.gov/pubmed/31729243
http://dx.doi.org/10.4155/bio-2019-0161
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