Streamlining the preparation of “endotoxin-free” ClearColi cell extract with autoinduction media for cell-free protein synthesis of the therapeutic protein crisantaspase

An “endotoxin-free” E. coli-based cell-free protein synthesis system has been reported to produce therapeutic proteins rapidly and on-demand. However, preparation of the most complex CFPS reagent – the cell extract – remains time-consuming and labor-intensive because of the relatively slow growth ki...

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Detalles Bibliográficos
Autores principales: Hunt, J. Porter, Zhao, Emily Long, Soltani, Mehran, Frei, Madison, Nelson, J. Andrew D., Bundy, Bradley C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6926305/
https://www.ncbi.nlm.nih.gov/pubmed/31890926
http://dx.doi.org/10.1016/j.synbio.2019.11.003

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6926305/
https://www.ncbi.nlm.nih.gov/pubmed/31890926
http://dx.doi.org/10.1016/j.synbio.2019.11.003

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