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Next-generation sequencing of dsRNA is greatly improved by treatment with the inexpensive denaturing reagent DMSO

dsRNA is the genetic material of important viruses and a key component of RNA interference-based immunity in eukaryotes. Previous studies have noted difficulties in determining the sequence of dsRNA molecules that have affected studies of immune function and estimates of viral diversity in nature. D...

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Detalles Bibliográficos
Autores principales: Wilcox, Alexander H., Delwart, Eric, Díaz-Muñoz, Samuel L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6927307/
https://www.ncbi.nlm.nih.gov/pubmed/31738702
http://dx.doi.org/10.1099/mgen.0.000315