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Next-generation sequencing of dsRNA is greatly improved by treatment with the inexpensive denaturing reagent DMSO
dsRNA is the genetic material of important viruses and a key component of RNA interference-based immunity in eukaryotes. Previous studies have noted difficulties in determining the sequence of dsRNA molecules that have affected studies of immune function and estimates of viral diversity in nature. D...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Microbiology Society
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6927307/ https://www.ncbi.nlm.nih.gov/pubmed/31738702 http://dx.doi.org/10.1099/mgen.0.000315 |