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Dead Cas Systems: Types, Principles, and Applications
The gene editing tool CRISPR-Cas has become the foundation for developing numerous molecular systems used in research and, increasingly, in medical practice. In particular, Cas proteins devoid of nucleolytic activity (dead Cas proteins; dCas) can be used to deliver functional cargo to programmed sit...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929090/ https://www.ncbi.nlm.nih.gov/pubmed/31801211 http://dx.doi.org/10.3390/ijms20236041 |
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author | Brezgin, Sergey Kostyusheva, Anastasiya Kostyushev, Dmitry Chulanov, Vladimir |
author_facet | Brezgin, Sergey Kostyusheva, Anastasiya Kostyushev, Dmitry Chulanov, Vladimir |
author_sort | Brezgin, Sergey |
collection | PubMed |
description | The gene editing tool CRISPR-Cas has become the foundation for developing numerous molecular systems used in research and, increasingly, in medical practice. In particular, Cas proteins devoid of nucleolytic activity (dead Cas proteins; dCas) can be used to deliver functional cargo to programmed sites in the genome. In this review, we describe current CRISPR systems used for developing different dCas-based molecular approaches and summarize their most significant applications. We conclude with comments on the state-of-art in the CRISPR field and future directions. |
format | Online Article Text |
id | pubmed-6929090 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69290902019-12-26 Dead Cas Systems: Types, Principles, and Applications Brezgin, Sergey Kostyusheva, Anastasiya Kostyushev, Dmitry Chulanov, Vladimir Int J Mol Sci Review The gene editing tool CRISPR-Cas has become the foundation for developing numerous molecular systems used in research and, increasingly, in medical practice. In particular, Cas proteins devoid of nucleolytic activity (dead Cas proteins; dCas) can be used to deliver functional cargo to programmed sites in the genome. In this review, we describe current CRISPR systems used for developing different dCas-based molecular approaches and summarize their most significant applications. We conclude with comments on the state-of-art in the CRISPR field and future directions. MDPI 2019-11-30 /pmc/articles/PMC6929090/ /pubmed/31801211 http://dx.doi.org/10.3390/ijms20236041 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Brezgin, Sergey Kostyusheva, Anastasiya Kostyushev, Dmitry Chulanov, Vladimir Dead Cas Systems: Types, Principles, and Applications |
title | Dead Cas Systems: Types, Principles, and Applications |
title_full | Dead Cas Systems: Types, Principles, and Applications |
title_fullStr | Dead Cas Systems: Types, Principles, and Applications |
title_full_unstemmed | Dead Cas Systems: Types, Principles, and Applications |
title_short | Dead Cas Systems: Types, Principles, and Applications |
title_sort | dead cas systems: types, principles, and applications |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929090/ https://www.ncbi.nlm.nih.gov/pubmed/31801211 http://dx.doi.org/10.3390/ijms20236041 |
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