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Dead Cas Systems: Types, Principles, and Applications

The gene editing tool CRISPR-Cas has become the foundation for developing numerous molecular systems used in research and, increasingly, in medical practice. In particular, Cas proteins devoid of nucleolytic activity (dead Cas proteins; dCas) can be used to deliver functional cargo to programmed sit...

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Detalles Bibliográficos
Autores principales: Brezgin, Sergey, Kostyusheva, Anastasiya, Kostyushev, Dmitry, Chulanov, Vladimir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929090/
https://www.ncbi.nlm.nih.gov/pubmed/31801211
http://dx.doi.org/10.3390/ijms20236041
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author Brezgin, Sergey
Kostyusheva, Anastasiya
Kostyushev, Dmitry
Chulanov, Vladimir
author_facet Brezgin, Sergey
Kostyusheva, Anastasiya
Kostyushev, Dmitry
Chulanov, Vladimir
author_sort Brezgin, Sergey
collection PubMed
description The gene editing tool CRISPR-Cas has become the foundation for developing numerous molecular systems used in research and, increasingly, in medical practice. In particular, Cas proteins devoid of nucleolytic activity (dead Cas proteins; dCas) can be used to deliver functional cargo to programmed sites in the genome. In this review, we describe current CRISPR systems used for developing different dCas-based molecular approaches and summarize their most significant applications. We conclude with comments on the state-of-art in the CRISPR field and future directions.
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spelling pubmed-69290902019-12-26 Dead Cas Systems: Types, Principles, and Applications Brezgin, Sergey Kostyusheva, Anastasiya Kostyushev, Dmitry Chulanov, Vladimir Int J Mol Sci Review The gene editing tool CRISPR-Cas has become the foundation for developing numerous molecular systems used in research and, increasingly, in medical practice. In particular, Cas proteins devoid of nucleolytic activity (dead Cas proteins; dCas) can be used to deliver functional cargo to programmed sites in the genome. In this review, we describe current CRISPR systems used for developing different dCas-based molecular approaches and summarize their most significant applications. We conclude with comments on the state-of-art in the CRISPR field and future directions. MDPI 2019-11-30 /pmc/articles/PMC6929090/ /pubmed/31801211 http://dx.doi.org/10.3390/ijms20236041 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Brezgin, Sergey
Kostyusheva, Anastasiya
Kostyushev, Dmitry
Chulanov, Vladimir
Dead Cas Systems: Types, Principles, and Applications
title Dead Cas Systems: Types, Principles, and Applications
title_full Dead Cas Systems: Types, Principles, and Applications
title_fullStr Dead Cas Systems: Types, Principles, and Applications
title_full_unstemmed Dead Cas Systems: Types, Principles, and Applications
title_short Dead Cas Systems: Types, Principles, and Applications
title_sort dead cas systems: types, principles, and applications
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929090/
https://www.ncbi.nlm.nih.gov/pubmed/31801211
http://dx.doi.org/10.3390/ijms20236041
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