Cargando…

Dual-Isotope SPECT Imaging with NIS Reporter Gene and Duramycin to Visualize Tumor Susceptibility to Oncolytic Virus Infection

Noninvasive dual-imaging methods that provide an early readout on tumor permissiveness to virus infection and tumor cell death could be valuable in optimizing development of oncolytic virotherapies. Here, we have used the sodium iodide symporter (NIS) and (125)I radiotracer to detect infection and r...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Lianwen, Suksanpaisan, Lukkana, Jiang, Huailei, DeGrado, Timothy R., Russell, Stephen J., Zhao, Ming, Peng, Kah-Whye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6931109/
https://www.ncbi.nlm.nih.gov/pubmed/31890867
http://dx.doi.org/10.1016/j.omto.2019.10.002
_version_ 1783483035545501696
author Zhang, Lianwen
Suksanpaisan, Lukkana
Jiang, Huailei
DeGrado, Timothy R.
Russell, Stephen J.
Zhao, Ming
Peng, Kah-Whye
author_facet Zhang, Lianwen
Suksanpaisan, Lukkana
Jiang, Huailei
DeGrado, Timothy R.
Russell, Stephen J.
Zhao, Ming
Peng, Kah-Whye
author_sort Zhang, Lianwen
collection PubMed
description Noninvasive dual-imaging methods that provide an early readout on tumor permissiveness to virus infection and tumor cell death could be valuable in optimizing development of oncolytic virotherapies. Here, we have used the sodium iodide symporter (NIS) and (125)I radiotracer to detect infection and replicative spread of an oncolytic vesicular stomatitis virus (VSV) in VSV-susceptible (MPC-11 tumor) versus VSV-resistant (CT26 tumor) tumors in BALB/c mice. In conjunction, tumor cell death was imaged simultaneously using technetium ((99m)Tc)-duramycin that binds phosphatidylethanolamine in apoptotic and necrotic cells. Dual-isotope single-photon emission computed tomography/computed tomography (SPECT/CT) imaging showed areas of virus infection (NIS and (125)I), which overlapped well with areas of tumor cell death ((99m)Tc-duramycin imaging) in susceptible tumors. Multiple infectious foci arose early in MPC-11 tumors, which rapidly expanded throughout the tumor parenchyma over time. There was a dose-dependent increase in numbers of infectious centers and (99m)Tc-duramycin-positive areas with viral dose. In contrast, NIS or duramycin signals were minimal in VSV-resistant CT26 tumors. Combinatorial use of NIS and (99m)Tc-duramycin SPECT imaging for simultaneous monitoring of oncolytic virotherapy (OV) spread and the presence or absence of treatment-associated cell death could be useful to guide development of combination treatment strategies to enhance therapeutic outcome.
format Online
Article
Text
id pubmed-6931109
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher American Society of Gene & Cell Therapy
record_format MEDLINE/PubMed
spelling pubmed-69311092019-12-30 Dual-Isotope SPECT Imaging with NIS Reporter Gene and Duramycin to Visualize Tumor Susceptibility to Oncolytic Virus Infection Zhang, Lianwen Suksanpaisan, Lukkana Jiang, Huailei DeGrado, Timothy R. Russell, Stephen J. Zhao, Ming Peng, Kah-Whye Mol Ther Oncolytics Article Noninvasive dual-imaging methods that provide an early readout on tumor permissiveness to virus infection and tumor cell death could be valuable in optimizing development of oncolytic virotherapies. Here, we have used the sodium iodide symporter (NIS) and (125)I radiotracer to detect infection and replicative spread of an oncolytic vesicular stomatitis virus (VSV) in VSV-susceptible (MPC-11 tumor) versus VSV-resistant (CT26 tumor) tumors in BALB/c mice. In conjunction, tumor cell death was imaged simultaneously using technetium ((99m)Tc)-duramycin that binds phosphatidylethanolamine in apoptotic and necrotic cells. Dual-isotope single-photon emission computed tomography/computed tomography (SPECT/CT) imaging showed areas of virus infection (NIS and (125)I), which overlapped well with areas of tumor cell death ((99m)Tc-duramycin imaging) in susceptible tumors. Multiple infectious foci arose early in MPC-11 tumors, which rapidly expanded throughout the tumor parenchyma over time. There was a dose-dependent increase in numbers of infectious centers and (99m)Tc-duramycin-positive areas with viral dose. In contrast, NIS or duramycin signals were minimal in VSV-resistant CT26 tumors. Combinatorial use of NIS and (99m)Tc-duramycin SPECT imaging for simultaneous monitoring of oncolytic virotherapy (OV) spread and the presence or absence of treatment-associated cell death could be useful to guide development of combination treatment strategies to enhance therapeutic outcome. American Society of Gene & Cell Therapy 2019-10-10 /pmc/articles/PMC6931109/ /pubmed/31890867 http://dx.doi.org/10.1016/j.omto.2019.10.002 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Zhang, Lianwen
Suksanpaisan, Lukkana
Jiang, Huailei
DeGrado, Timothy R.
Russell, Stephen J.
Zhao, Ming
Peng, Kah-Whye
Dual-Isotope SPECT Imaging with NIS Reporter Gene and Duramycin to Visualize Tumor Susceptibility to Oncolytic Virus Infection
title Dual-Isotope SPECT Imaging with NIS Reporter Gene and Duramycin to Visualize Tumor Susceptibility to Oncolytic Virus Infection
title_full Dual-Isotope SPECT Imaging with NIS Reporter Gene and Duramycin to Visualize Tumor Susceptibility to Oncolytic Virus Infection
title_fullStr Dual-Isotope SPECT Imaging with NIS Reporter Gene and Duramycin to Visualize Tumor Susceptibility to Oncolytic Virus Infection
title_full_unstemmed Dual-Isotope SPECT Imaging with NIS Reporter Gene and Duramycin to Visualize Tumor Susceptibility to Oncolytic Virus Infection
title_short Dual-Isotope SPECT Imaging with NIS Reporter Gene and Duramycin to Visualize Tumor Susceptibility to Oncolytic Virus Infection
title_sort dual-isotope spect imaging with nis reporter gene and duramycin to visualize tumor susceptibility to oncolytic virus infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6931109/
https://www.ncbi.nlm.nih.gov/pubmed/31890867
http://dx.doi.org/10.1016/j.omto.2019.10.002
work_keys_str_mv AT zhanglianwen dualisotopespectimagingwithnisreportergeneandduramycintovisualizetumorsusceptibilitytooncolyticvirusinfection
AT suksanpaisanlukkana dualisotopespectimagingwithnisreportergeneandduramycintovisualizetumorsusceptibilitytooncolyticvirusinfection
AT jianghuailei dualisotopespectimagingwithnisreportergeneandduramycintovisualizetumorsusceptibilitytooncolyticvirusinfection
AT degradotimothyr dualisotopespectimagingwithnisreportergeneandduramycintovisualizetumorsusceptibilitytooncolyticvirusinfection
AT russellstephenj dualisotopespectimagingwithnisreportergeneandduramycintovisualizetumorsusceptibilitytooncolyticvirusinfection
AT zhaoming dualisotopespectimagingwithnisreportergeneandduramycintovisualizetumorsusceptibilitytooncolyticvirusinfection
AT pengkahwhye dualisotopespectimagingwithnisreportergeneandduramycintovisualizetumorsusceptibilitytooncolyticvirusinfection