AlleleProfileR: A versatile tool to identify and profile sequence variants in edited genomes

Gene editing strategies, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR/Cas9), are revolutionizing biology. However, quantitative and sensitive detection of targeted mutations a...

Descripción completa

Detalles Bibliográficos
Autores principales: Bruyneel, Arne A. N., Colas, Alexandre R., Karakikes, Ioannis, Mercola, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6932767/
https://www.ncbi.nlm.nih.gov/pubmed/31877162
http://dx.doi.org/10.1371/journal.pone.0226694
Descripción
Sumario:Gene editing strategies, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR/Cas9), are revolutionizing biology. However, quantitative and sensitive detection of targeted mutations are required to evaluate and quantify the genome editing outcomes. Here we present AlleleProfileR, a new analysis tool, written in a combination of R and C++, with the ability to batch process the sequence analysis of large and complex genome editing experiments, including the recently developed base editing technologies.

Ejemplares similares