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Screening for functional IRESes using α-complementation system of β-galactosidase in Pichia pastoris
BACKGROUND: Pichia pastoris is becoming a promising chassis cell for metabolic engineering and synthetic biology after its whole genome and transcriptome sequenced. However, the current systems for multigene co-expression in P. pastoris are not efficient. The internal ribosome entry site (IRES) has...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933714/ https://www.ncbi.nlm.nih.gov/pubmed/31890028 http://dx.doi.org/10.1186/s13068-019-1640-3 |