Rapid Tagging of Human Proteins with Fluorescent Reporters by Genome Engineering using Double‐Stranded DNA Donors

Tagging proteins with fluorescent reporters such as green fluorescent protein (GFP) is a powerful method to determine protein localization, especially when proteins are tagged in the endogenous context to preserve native genomic regulation. However, insertion of fluorescent reporters into the genome...

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Detalles Bibliográficos
Autores principales: Paix, Alexandre, Rasoloson, Dominique, Folkmann, Andrew, Seydoux, Geraldine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Updated Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6935516/
https://www.ncbi.nlm.nih.gov/pubmed/31710422
http://dx.doi.org/10.1002/cpmb.102

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6935516/
https://www.ncbi.nlm.nih.gov/pubmed/31710422
http://dx.doi.org/10.1002/cpmb.102

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