Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics
Optogenetics, which uses visible light to control the cells genetically modified with light-gated ion channels, is a powerful tool for precise deconstruction of neural circuitry with neuron-subtype specificity. However, due to limited tissue penetration of visible light, invasive craniotomy and intr...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6936518/ https://www.ncbi.nlm.nih.gov/pubmed/31811026 http://dx.doi.org/10.1073/pnas.1914387116 |
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author | Wu, Xiang Zhu, Xingjun Chong, Paul Liu, Junlang Andre, Louis N. Ong, Kyrstyn S. Brinson, Kenneth Mahdi, Ali I. Li, Jiachen Fenno, Lief E. Wang, Huiliang Hong, Guosong |
author_facet | Wu, Xiang Zhu, Xingjun Chong, Paul Liu, Junlang Andre, Louis N. Ong, Kyrstyn S. Brinson, Kenneth Mahdi, Ali I. Li, Jiachen Fenno, Lief E. Wang, Huiliang Hong, Guosong |
author_sort | Wu, Xiang |
collection | PubMed |
description | Optogenetics, which uses visible light to control the cells genetically modified with light-gated ion channels, is a powerful tool for precise deconstruction of neural circuitry with neuron-subtype specificity. However, due to limited tissue penetration of visible light, invasive craniotomy and intracranial implantation of tethered optical fibers are usually required for in vivo optogenetic modulation. Here we report mechanoluminescent nanoparticles that can act as local light sources in the brain when triggered by brain-penetrant focused ultrasound (FUS) through intact scalp and skull. Mechanoluminescent nanoparticles can be delivered into the blood circulation via i.v. injection, recharged by 400-nm photoexcitation light in superficial blood vessels during circulation, and turned on by FUS to emit 470-nm light repetitively in the intact brain for optogenetic stimulation. Unlike the conventional “outside-in” approaches of optogenetics with fiber implantation, our method provides an “inside-out” approach to deliver nanoscopic light emitters via the intrinsic circulatory system and switch them on and off at any time and location of interest in the brain without extravasation through a minimally invasive ultrasound interface. |
format | Online Article Text |
id | pubmed-6936518 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-69365182019-12-31 Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics Wu, Xiang Zhu, Xingjun Chong, Paul Liu, Junlang Andre, Louis N. Ong, Kyrstyn S. Brinson, Kenneth Mahdi, Ali I. Li, Jiachen Fenno, Lief E. Wang, Huiliang Hong, Guosong Proc Natl Acad Sci U S A Physical Sciences Optogenetics, which uses visible light to control the cells genetically modified with light-gated ion channels, is a powerful tool for precise deconstruction of neural circuitry with neuron-subtype specificity. However, due to limited tissue penetration of visible light, invasive craniotomy and intracranial implantation of tethered optical fibers are usually required for in vivo optogenetic modulation. Here we report mechanoluminescent nanoparticles that can act as local light sources in the brain when triggered by brain-penetrant focused ultrasound (FUS) through intact scalp and skull. Mechanoluminescent nanoparticles can be delivered into the blood circulation via i.v. injection, recharged by 400-nm photoexcitation light in superficial blood vessels during circulation, and turned on by FUS to emit 470-nm light repetitively in the intact brain for optogenetic stimulation. Unlike the conventional “outside-in” approaches of optogenetics with fiber implantation, our method provides an “inside-out” approach to deliver nanoscopic light emitters via the intrinsic circulatory system and switch them on and off at any time and location of interest in the brain without extravasation through a minimally invasive ultrasound interface. National Academy of Sciences 2019-12-26 2019-12-06 /pmc/articles/PMC6936518/ /pubmed/31811026 http://dx.doi.org/10.1073/pnas.1914387116 Text en Copyright © 2019 the Author(s). Published by PNAS. http://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Physical Sciences Wu, Xiang Zhu, Xingjun Chong, Paul Liu, Junlang Andre, Louis N. Ong, Kyrstyn S. Brinson, Kenneth Mahdi, Ali I. Li, Jiachen Fenno, Lief E. Wang, Huiliang Hong, Guosong Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics |
title | Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics |
title_full | Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics |
title_fullStr | Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics |
title_full_unstemmed | Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics |
title_short | Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics |
title_sort | sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics |
topic | Physical Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6936518/ https://www.ncbi.nlm.nih.gov/pubmed/31811026 http://dx.doi.org/10.1073/pnas.1914387116 |
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