Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics

Optogenetics, which uses visible light to control the cells genetically modified with light-gated ion channels, is a powerful tool for precise deconstruction of neural circuitry with neuron-subtype specificity. However, due to limited tissue penetration of visible light, invasive craniotomy and intr...

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Autores principales: Wu, Xiang, Zhu, Xingjun, Chong, Paul, Liu, Junlang, Andre, Louis N., Ong, Kyrstyn S., Brinson, Kenneth, Mahdi, Ali I., Li, Jiachen, Fenno, Lief E., Wang, Huiliang, Hong, Guosong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6936518/
https://www.ncbi.nlm.nih.gov/pubmed/31811026
http://dx.doi.org/10.1073/pnas.1914387116
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author Wu, Xiang
Zhu, Xingjun
Chong, Paul
Liu, Junlang
Andre, Louis N.
Ong, Kyrstyn S.
Brinson, Kenneth
Mahdi, Ali I.
Li, Jiachen
Fenno, Lief E.
Wang, Huiliang
Hong, Guosong
author_facet Wu, Xiang
Zhu, Xingjun
Chong, Paul
Liu, Junlang
Andre, Louis N.
Ong, Kyrstyn S.
Brinson, Kenneth
Mahdi, Ali I.
Li, Jiachen
Fenno, Lief E.
Wang, Huiliang
Hong, Guosong
author_sort Wu, Xiang
collection PubMed
description Optogenetics, which uses visible light to control the cells genetically modified with light-gated ion channels, is a powerful tool for precise deconstruction of neural circuitry with neuron-subtype specificity. However, due to limited tissue penetration of visible light, invasive craniotomy and intracranial implantation of tethered optical fibers are usually required for in vivo optogenetic modulation. Here we report mechanoluminescent nanoparticles that can act as local light sources in the brain when triggered by brain-penetrant focused ultrasound (FUS) through intact scalp and skull. Mechanoluminescent nanoparticles can be delivered into the blood circulation via i.v. injection, recharged by 400-nm photoexcitation light in superficial blood vessels during circulation, and turned on by FUS to emit 470-nm light repetitively in the intact brain for optogenetic stimulation. Unlike the conventional “outside-in” approaches of optogenetics with fiber implantation, our method provides an “inside-out” approach to deliver nanoscopic light emitters via the intrinsic circulatory system and switch them on and off at any time and location of interest in the brain without extravasation through a minimally invasive ultrasound interface.
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spelling pubmed-69365182019-12-31 Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics Wu, Xiang Zhu, Xingjun Chong, Paul Liu, Junlang Andre, Louis N. Ong, Kyrstyn S. Brinson, Kenneth Mahdi, Ali I. Li, Jiachen Fenno, Lief E. Wang, Huiliang Hong, Guosong Proc Natl Acad Sci U S A Physical Sciences Optogenetics, which uses visible light to control the cells genetically modified with light-gated ion channels, is a powerful tool for precise deconstruction of neural circuitry with neuron-subtype specificity. However, due to limited tissue penetration of visible light, invasive craniotomy and intracranial implantation of tethered optical fibers are usually required for in vivo optogenetic modulation. Here we report mechanoluminescent nanoparticles that can act as local light sources in the brain when triggered by brain-penetrant focused ultrasound (FUS) through intact scalp and skull. Mechanoluminescent nanoparticles can be delivered into the blood circulation via i.v. injection, recharged by 400-nm photoexcitation light in superficial blood vessels during circulation, and turned on by FUS to emit 470-nm light repetitively in the intact brain for optogenetic stimulation. Unlike the conventional “outside-in” approaches of optogenetics with fiber implantation, our method provides an “inside-out” approach to deliver nanoscopic light emitters via the intrinsic circulatory system and switch them on and off at any time and location of interest in the brain without extravasation through a minimally invasive ultrasound interface. National Academy of Sciences 2019-12-26 2019-12-06 /pmc/articles/PMC6936518/ /pubmed/31811026 http://dx.doi.org/10.1073/pnas.1914387116 Text en Copyright © 2019 the Author(s). Published by PNAS. http://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Physical Sciences
Wu, Xiang
Zhu, Xingjun
Chong, Paul
Liu, Junlang
Andre, Louis N.
Ong, Kyrstyn S.
Brinson, Kenneth
Mahdi, Ali I.
Li, Jiachen
Fenno, Lief E.
Wang, Huiliang
Hong, Guosong
Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics
title Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics
title_full Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics
title_fullStr Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics
title_full_unstemmed Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics
title_short Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics
title_sort sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics
topic Physical Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6936518/
https://www.ncbi.nlm.nih.gov/pubmed/31811026
http://dx.doi.org/10.1073/pnas.1914387116
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