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The effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of L-phenylalanine-ammonia-lyase

The effect of three types of polysaccharides (agar-agar, carrageenan, hydroxypropyl methylcellulose) on the activity and stability during storage at given temperature conditions of the enzyme preparation L-phenylalanine ammonia-lyase was studied. It was found that the most suitable storage temperatu...

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Detalles Bibliográficos
Autores principales: Babich, Olga, Dyshlyuk, Lyubov, Noskova, Svetlana, Prosekov, Alexander, Ivanova, Svetlana, Pavsky, Valery
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6938834/
https://www.ncbi.nlm.nih.gov/pubmed/31909265
http://dx.doi.org/10.1016/j.heliyon.2019.e03096
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author Babich, Olga
Dyshlyuk, Lyubov
Noskova, Svetlana
Prosekov, Alexander
Ivanova, Svetlana
Pavsky, Valery
author_facet Babich, Olga
Dyshlyuk, Lyubov
Noskova, Svetlana
Prosekov, Alexander
Ivanova, Svetlana
Pavsky, Valery
author_sort Babich, Olga
collection PubMed
description The effect of three types of polysaccharides (agar-agar, carrageenan, hydroxypropyl methylcellulose) on the activity and stability during storage at given temperature conditions of the enzyme preparation L-phenylalanine ammonia-lyase was studied. It was found that the most suitable storage temperature for encapsulated L-phenylalanine-ammonia-lyase is room temperature up to 25 °C for all samples of capsules from plant polysaccharides. Samples of capsules with agar-agar and hydroxypropyl methylcellulose under different temperature conditions inhibited the decrease in enzyme activity, which in other samples of capsules reached 90% in 6 months of storage. In samples of capsules with carrageenan at temperatures of 4 °C and 30 °C, there was a significant decrease in the activity of the enzyme preparation. Selection of capsule samples from plant polysaccharides suitable for L-phenylalanine-ammonia-lyase replacement therapy is done after studying the mechanisms of capsule destruction under conditions close to the conditions of the gastrointestinal tract, to which the next stage of our research will be devoted.
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spelling pubmed-69388342020-01-06 The effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of L-phenylalanine-ammonia-lyase Babich, Olga Dyshlyuk, Lyubov Noskova, Svetlana Prosekov, Alexander Ivanova, Svetlana Pavsky, Valery Heliyon Article The effect of three types of polysaccharides (agar-agar, carrageenan, hydroxypropyl methylcellulose) on the activity and stability during storage at given temperature conditions of the enzyme preparation L-phenylalanine ammonia-lyase was studied. It was found that the most suitable storage temperature for encapsulated L-phenylalanine-ammonia-lyase is room temperature up to 25 °C for all samples of capsules from plant polysaccharides. Samples of capsules with agar-agar and hydroxypropyl methylcellulose under different temperature conditions inhibited the decrease in enzyme activity, which in other samples of capsules reached 90% in 6 months of storage. In samples of capsules with carrageenan at temperatures of 4 °C and 30 °C, there was a significant decrease in the activity of the enzyme preparation. Selection of capsule samples from plant polysaccharides suitable for L-phenylalanine-ammonia-lyase replacement therapy is done after studying the mechanisms of capsule destruction under conditions close to the conditions of the gastrointestinal tract, to which the next stage of our research will be devoted. Elsevier 2019-12-27 /pmc/articles/PMC6938834/ /pubmed/31909265 http://dx.doi.org/10.1016/j.heliyon.2019.e03096 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Babich, Olga
Dyshlyuk, Lyubov
Noskova, Svetlana
Prosekov, Alexander
Ivanova, Svetlana
Pavsky, Valery
The effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of L-phenylalanine-ammonia-lyase
title The effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of L-phenylalanine-ammonia-lyase
title_full The effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of L-phenylalanine-ammonia-lyase
title_fullStr The effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of L-phenylalanine-ammonia-lyase
title_full_unstemmed The effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of L-phenylalanine-ammonia-lyase
title_short The effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of L-phenylalanine-ammonia-lyase
title_sort effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of l-phenylalanine-ammonia-lyase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6938834/
https://www.ncbi.nlm.nih.gov/pubmed/31909265
http://dx.doi.org/10.1016/j.heliyon.2019.e03096
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