Detection and quantification of Erysipelothrix rhusiopathiae in blood from infected chickens – addressing challenges with detection of DNA from infectious agents in host species with nucleated red blood cells

PURPOSE: The present study aimed to establish pretreatment protocols as well as real-time and droplet digital polymerase chain reaction (PCR) methodologies to detect and quantify Erysipelothrix rhusiopathiae (ER) DNA in blood samples from infected chickens, as tools for routine diagnostics and monit...

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Detalles Bibliográficos
Autores principales: Wattrang, Eva, Jäderblom, Victoria, Jinnerot, Tomas, Eriksson, Helena, Bagge, Elisabeth, Persson, Maria, Dalgaard, Tina Sørensen, Söderlund, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6939158/
https://www.ncbi.nlm.nih.gov/pubmed/31172912
http://dx.doi.org/10.1099/jmm.0.001016

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6939158/
https://www.ncbi.nlm.nih.gov/pubmed/31172912
http://dx.doi.org/10.1099/jmm.0.001016

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