Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1

ATOX1 is a copper chaperone involved in intracellular copper homeostasis, cell proliferation, and tumor progression. To investigate the physiologically relevant molecular mechanism of ATOX1 by using imaging-based approaches, we genetically modified ATOX1 in H1 hESCs to express mCherry-ATOX1 fusion p...

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Autores principales: Wen, Meng-Hsuan, Xie, Xihong, Tu, Jian, Lee, Dung-Fang, Chen, Tai-Yen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6939864/
https://www.ncbi.nlm.nih.gov/pubmed/31704540
http://dx.doi.org/10.1016/j.scr.2019.101631
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author Wen, Meng-Hsuan
Xie, Xihong
Tu, Jian
Lee, Dung-Fang
Chen, Tai-Yen
author_facet Wen, Meng-Hsuan
Xie, Xihong
Tu, Jian
Lee, Dung-Fang
Chen, Tai-Yen
author_sort Wen, Meng-Hsuan
collection PubMed
description ATOX1 is a copper chaperone involved in intracellular copper homeostasis, cell proliferation, and tumor progression. To investigate the physiologically relevant molecular mechanism of ATOX1 by using imaging-based approaches, we genetically modified ATOX1 in H1 hESCs to express mCherry-ATOX1 fusion protein under endogenous regulatory machinery. The fluorescence engineered hESC clone maintains characteristic stem cell features and can differentiate to all three germ layers, serving as a unique tool to dissect the role of ATOX1 in various cellular processes.
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spelling pubmed-69398642020-01-02 Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1 Wen, Meng-Hsuan Xie, Xihong Tu, Jian Lee, Dung-Fang Chen, Tai-Yen Stem Cell Res Article ATOX1 is a copper chaperone involved in intracellular copper homeostasis, cell proliferation, and tumor progression. To investigate the physiologically relevant molecular mechanism of ATOX1 by using imaging-based approaches, we genetically modified ATOX1 in H1 hESCs to express mCherry-ATOX1 fusion protein under endogenous regulatory machinery. The fluorescence engineered hESC clone maintains characteristic stem cell features and can differentiate to all three germ layers, serving as a unique tool to dissect the role of ATOX1 in various cellular processes. 2019-10-16 2019-12 /pmc/articles/PMC6939864/ /pubmed/31704540 http://dx.doi.org/10.1016/j.scr.2019.101631 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Wen, Meng-Hsuan
Xie, Xihong
Tu, Jian
Lee, Dung-Fang
Chen, Tai-Yen
Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1
title Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1
title_full Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1
title_fullStr Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1
title_full_unstemmed Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1
title_short Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1
title_sort generation of a genetically modified human embryonic stem cells expressing fluorescence tagged atox1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6939864/
https://www.ncbi.nlm.nih.gov/pubmed/31704540
http://dx.doi.org/10.1016/j.scr.2019.101631
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