A Single-Turnover Kinetic Study of DNA Demethylation Catalyzed by Fe(II)/α-Ketoglutarate-Dependent Dioxygenase AlkB

AlkB is a Fe(II)/α-ketoglutarate-dependent dioxygenase that repairs some alkylated bases of DNA and RNA in Escherichia coli. In the course of catalysis, oxidation of a co-substrate (α-ketoglutarate, αKG) leads to the formation of a highly reactive ‘oxyferryl’ enzyme-bound intermediate, Fe(IV) = O, e...

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Detalles Bibliográficos
Autores principales: Kanazhevskaya, Lyubov Yu., Alekseeva, Irina V., Fedorova, Olga S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943663/
https://www.ncbi.nlm.nih.gov/pubmed/31847292
http://dx.doi.org/10.3390/molecules24244576

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943663/
https://www.ncbi.nlm.nih.gov/pubmed/31847292
http://dx.doi.org/10.3390/molecules24244576

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