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Enhanced efficiency in isolation and expansion of hAMSCs via dual enzyme digestion and micro-carrier

A two-stage method of obtaining viable human amniotic stem cells (hAMSCs) in large-scale is described. First, human amniotic stem cells are isolated via dual enzyme (collagenase II and DNAase I) digestion. Next, relying on a culture of the cells from porous chitosan-based microspheres in vitro, high...

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Detalles Bibliográficos
Autores principales: Gohi, Bi Foua Claude Alain, Liu, Xue-Ying, Zeng, Hong-Yan, Xu, Sheng, Ake, Kouassi Marius Honore, Cao, Xiao-Ju, Zou, Kai-Min, Namulondo, Sheila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6945441/
https://www.ncbi.nlm.nih.gov/pubmed/31921407
http://dx.doi.org/10.1186/s13578-019-0367-y