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Systematic assessment of commercially available low-input miRNA library preparation kits

High-throughput sequencing is increasingly favoured to assay the presence and abundance of microRNAs (miRNAs) in biological samples, even from low RNA amounts, and a number of commercial vendors now offer kits that allow miRNA sequencing from sub-nanogram (ng) inputs. Although biases introduced duri...

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Detalles Bibliográficos
Autores principales: Heinicke, Fatima, Zhong, Xiangfu, Zucknick, Manuela, Breidenbach, Johannes, Sundaram, Arvind Y. M., T. Flåm, Siri, Leithaug, Magnus, Dalland, Marianne, Farmer, Andrew, Henderson, Jordana M., Hussong, Melanie A., Moll, Pamela, Nguyen, Loan, McNulty, Amanda, Shaffer, Jonathan M., Shore, Sabrina, Yip, Hoichong Karen, Vitkovska, Jana, Rayner, Simon, Lie, Benedicte A, Gilfillan, Gregor D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948978/
https://www.ncbi.nlm.nih.gov/pubmed/31559901
http://dx.doi.org/10.1080/15476286.2019.1667741