Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies

Spatially-controlled cell adhesion on electron microscopy (EM) supports remains a bottleneck in specimen preparation for cellular cryo-electron tomography. Here, we describe contactless and mask-free photo-micropatterning of EM grids for site-specific deposition of extracellular matrix (ECM)-related...

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Detalles Bibliográficos
Autores principales: Toro-Nahuelpan, Mauricio, Zagoriy, Ievgeniia, Senger, Fabrice, Blanchoin, Laurent, Théry, Manuel, Mahamid, Julia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949126/
https://www.ncbi.nlm.nih.gov/pubmed/31740821
http://dx.doi.org/10.1038/s41592-019-0630-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949126/
https://www.ncbi.nlm.nih.gov/pubmed/31740821
http://dx.doi.org/10.1038/s41592-019-0630-5

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