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Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies
Spatially-controlled cell adhesion on electron microscopy (EM) supports remains a bottleneck in specimen preparation for cellular cryo-electron tomography. Here, we describe contactless and mask-free photo-micropatterning of EM grids for site-specific deposition of extracellular matrix (ECM)-related...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949126/ https://www.ncbi.nlm.nih.gov/pubmed/31740821 http://dx.doi.org/10.1038/s41592-019-0630-5 |
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author | Toro-Nahuelpan, Mauricio Zagoriy, Ievgeniia Senger, Fabrice Blanchoin, Laurent Théry, Manuel Mahamid, Julia |
author_facet | Toro-Nahuelpan, Mauricio Zagoriy, Ievgeniia Senger, Fabrice Blanchoin, Laurent Théry, Manuel Mahamid, Julia |
author_sort | Toro-Nahuelpan, Mauricio |
collection | PubMed |
description | Spatially-controlled cell adhesion on electron microscopy (EM) supports remains a bottleneck in specimen preparation for cellular cryo-electron tomography. Here, we describe contactless and mask-free photo-micropatterning of EM grids for site-specific deposition of extracellular matrix (ECM)-related proteins. We attained refined cell positioning for micromachining by cryo-focused ion beam milling. Complex micropatterns generated predictable intracellular organization, allowing direct correlation between cell architecture and in-cell 3D-structural characterization of the underlying molecular machinery. |
format | Online Article Text |
id | pubmed-6949126 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
record_format | MEDLINE/PubMed |
spelling | pubmed-69491262020-05-18 Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies Toro-Nahuelpan, Mauricio Zagoriy, Ievgeniia Senger, Fabrice Blanchoin, Laurent Théry, Manuel Mahamid, Julia Nat Methods Article Spatially-controlled cell adhesion on electron microscopy (EM) supports remains a bottleneck in specimen preparation for cellular cryo-electron tomography. Here, we describe contactless and mask-free photo-micropatterning of EM grids for site-specific deposition of extracellular matrix (ECM)-related proteins. We attained refined cell positioning for micromachining by cryo-focused ion beam milling. Complex micropatterns generated predictable intracellular organization, allowing direct correlation between cell architecture and in-cell 3D-structural characterization of the underlying molecular machinery. 2020-01-01 2019-11-18 /pmc/articles/PMC6949126/ /pubmed/31740821 http://dx.doi.org/10.1038/s41592-019-0630-5 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Toro-Nahuelpan, Mauricio Zagoriy, Ievgeniia Senger, Fabrice Blanchoin, Laurent Théry, Manuel Mahamid, Julia Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies |
title | Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies |
title_full | Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies |
title_fullStr | Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies |
title_full_unstemmed | Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies |
title_short | Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies |
title_sort | tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949126/ https://www.ncbi.nlm.nih.gov/pubmed/31740821 http://dx.doi.org/10.1038/s41592-019-0630-5 |
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