Cas9/gRNA-mediated genome editing of yeast mitochondria and Chlamydomonas chloroplasts

We present a new approach to edit both mitochondrial and chloroplast genomes. Organelles have been considered off-limits to CRISPR due to their impermeability to most RNA and DNA. This has prevented applications of Cas9/gRNA-mediated genome editing in organelles while the tool has been widely used f...

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Detalles Bibliográficos
Autores principales: Yoo, Byung-Chun, Yadav, Narendra S., Orozco, Emil M., Sakai, Hajime
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6951285/
https://www.ncbi.nlm.nih.gov/pubmed/31934513
http://dx.doi.org/10.7717/peerj.8362

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6951285/
https://www.ncbi.nlm.nih.gov/pubmed/31934513
http://dx.doi.org/10.7717/peerj.8362

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