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Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis
Technical problems intrinsic to the purification of preribosome intermediates have limited our understanding of ribosome biosynthesis in humans. Addressing this issue is important given the implication of this biological process in human disease. Here we report a preribosome purification and tagging...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952385/ https://www.ncbi.nlm.nih.gov/pubmed/31919354 http://dx.doi.org/10.1038/s41467-019-13990-w |
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author | Nieto, Blanca Gaspar, Sonia G. Moriggi, Giulia Pestov, Dimitri G. Bustelo, Xosé R. Dosil, Mercedes |
author_facet | Nieto, Blanca Gaspar, Sonia G. Moriggi, Giulia Pestov, Dimitri G. Bustelo, Xosé R. Dosil, Mercedes |
author_sort | Nieto, Blanca |
collection | PubMed |
description | Technical problems intrinsic to the purification of preribosome intermediates have limited our understanding of ribosome biosynthesis in humans. Addressing this issue is important given the implication of this biological process in human disease. Here we report a preribosome purification and tagging strategy that overcomes some of the existing technical difficulties. Using these tools, we find that the pre-40S precursors go through two distinct maturation phases inside the nucleolus and follow a regulatory step that precedes late maturation in the cytoplasm. This regulatory step entails the intertwined actions of both PARN (a metazoan-specific ribonuclease) and RRP12 (a phylogenetically conserved 40S biogenesis factor that has acquired additional functional features in higher eukaryotes). Together, these results demonstrate the usefulness of this purification method for the dissection of ribosome biogenesis in human cells. They also identify distinct maturation stages and metazoan-specific regulatory mechanisms involved in the generation of the human 40S ribosomal subunit. |
format | Online Article Text |
id | pubmed-6952385 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69523852020-01-13 Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis Nieto, Blanca Gaspar, Sonia G. Moriggi, Giulia Pestov, Dimitri G. Bustelo, Xosé R. Dosil, Mercedes Nat Commun Article Technical problems intrinsic to the purification of preribosome intermediates have limited our understanding of ribosome biosynthesis in humans. Addressing this issue is important given the implication of this biological process in human disease. Here we report a preribosome purification and tagging strategy that overcomes some of the existing technical difficulties. Using these tools, we find that the pre-40S precursors go through two distinct maturation phases inside the nucleolus and follow a regulatory step that precedes late maturation in the cytoplasm. This regulatory step entails the intertwined actions of both PARN (a metazoan-specific ribonuclease) and RRP12 (a phylogenetically conserved 40S biogenesis factor that has acquired additional functional features in higher eukaryotes). Together, these results demonstrate the usefulness of this purification method for the dissection of ribosome biogenesis in human cells. They also identify distinct maturation stages and metazoan-specific regulatory mechanisms involved in the generation of the human 40S ribosomal subunit. Nature Publishing Group UK 2020-01-09 /pmc/articles/PMC6952385/ /pubmed/31919354 http://dx.doi.org/10.1038/s41467-019-13990-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Nieto, Blanca Gaspar, Sonia G. Moriggi, Giulia Pestov, Dimitri G. Bustelo, Xosé R. Dosil, Mercedes Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis |
title | Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis |
title_full | Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis |
title_fullStr | Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis |
title_full_unstemmed | Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis |
title_short | Identification of distinct maturation steps involved in human 40S ribosomal subunit biosynthesis |
title_sort | identification of distinct maturation steps involved in human 40s ribosomal subunit biosynthesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952385/ https://www.ncbi.nlm.nih.gov/pubmed/31919354 http://dx.doi.org/10.1038/s41467-019-13990-w |
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