Cloning, Optimization of Periplasmic Expression and Purification of Recombinant Granulocyte Macrophage-Stimulating Factor in Escherichia coli BL21 (DE3)

BACKGROUND: Molgramostim, a nonglycosylated version of recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF), can be produced in a high level by Escherichia coli. However, overexpression of GM-CSF in bacterial cells usually leads to formation of inclusion bodies and insoluble protein...

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Detalles Bibliográficos
Autores principales: Taherian, Elham, Mohammadi, Elmira, Jahanian-Najafabadi, Ali, Moazen, Fatemeh, Akbari, Vajihe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2019
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952766/
https://www.ncbi.nlm.nih.gov/pubmed/32002394
http://dx.doi.org/10.4103/abr.abr_166_19

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952766/
https://www.ncbi.nlm.nih.gov/pubmed/32002394
http://dx.doi.org/10.4103/abr.abr_166_19

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