Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency

Versatile and precise genome modifications are needed for a wider range of adoptive cellular therapies(1–5). Here we report two improvements that increase the efficiency of CRISPR-Cas9-based genome editing in clinically relevant primary cell types. Truncated Cas9 target sequences (tCTS) added at the...

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Detalles Bibliográficos
Autores principales: Nguyen, David N., Roth, Theodore L., Li, P. Jonathan, Chen, Peixin Amy, Apathy, Ryan, Mamedov, Murad R., Vo, Linda T., Tobin, Victoria R., Goodman, Daniel, Shifrut, Eric, Bluestone, Jeffrey A., Puck, Jennifer M., Szoka, Francis C., Marson, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6954310/
https://www.ncbi.nlm.nih.gov/pubmed/31819258
http://dx.doi.org/10.1038/s41587-019-0325-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6954310/
https://www.ncbi.nlm.nih.gov/pubmed/31819258
http://dx.doi.org/10.1038/s41587-019-0325-6

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