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Targeted insertional mutagenesis libraries for deep domain insertion profiling

Domain recombination is a key principle in protein evolution and protein engineering, but inserting a donor domain into every position of a target protein is not easily experimentally accessible. Most contemporary domain insertion profiling approaches rely on DNA transposons, which are constrained b...

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Detalles Bibliográficos
Autores principales: Coyote-Maestas, Willow, Nedrud, David, Okorafor, Steffan, He, Yungui, Schmidt, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6954442/
https://www.ncbi.nlm.nih.gov/pubmed/31745561
http://dx.doi.org/10.1093/nar/gkz1110