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Targeted insertional mutagenesis libraries for deep domain insertion profiling
Domain recombination is a key principle in protein evolution and protein engineering, but inserting a donor domain into every position of a target protein is not easily experimentally accessible. Most contemporary domain insertion profiling approaches rely on DNA transposons, which are constrained b...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6954442/ https://www.ncbi.nlm.nih.gov/pubmed/31745561 http://dx.doi.org/10.1093/nar/gkz1110 |