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Custom selected reference genes outperform pre-defined reference genes in transcriptomic analysis

BACKGROUND: RNA sequencing allows the measuring of gene expression at a resolution unmet by expression arrays or RT-qPCR. It is however necessary to normalize sequencing data by library size, transcript size and composition, among other factors, before comparing expression levels. The use of interna...

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Detalles Bibliográficos
Autores principales: dos Santos, Karen Cristine Gonçalves, Desgagné-Penix, Isabel, Germain, Hugo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6954607/
https://www.ncbi.nlm.nih.gov/pubmed/31924161
http://dx.doi.org/10.1186/s12864-019-6426-2