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Use of in-gel peroxidase assay for cytochrome c to visualize mitochondrial complexes III and IV

The in-gel activity assay (IGA) is a powerful technique that uses enzymatic activity and compares intensities of detected bands in mitochondrial respiratory chain supercomplexes, and it is applicable to eukaryotic organisms. However, no IGA has been established for complex III because of the difficu...

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Detalles Bibliográficos
Autores principales: Hara, Tsukasa, Shibata, Yuma, Amagai, Ryosuke, Okado-Matsumoto, Ayako
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6955206/
https://www.ncbi.nlm.nih.gov/pubmed/31852667
http://dx.doi.org/10.1242/bio.047936