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Use of in-gel peroxidase assay for cytochrome c to visualize mitochondrial complexes III and IV
The in-gel activity assay (IGA) is a powerful technique that uses enzymatic activity and compares intensities of detected bands in mitochondrial respiratory chain supercomplexes, and it is applicable to eukaryotic organisms. However, no IGA has been established for complex III because of the difficu...
Autores principales: | Hara, Tsukasa, Shibata, Yuma, Amagai, Ryosuke, Okado-Matsumoto, Ayako |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6955206/ https://www.ncbi.nlm.nih.gov/pubmed/31852667 http://dx.doi.org/10.1242/bio.047936 |
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