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Unspecific DNA recombination in AdipoqCre-ER(T2) – mediated knockout approaches in transgenic mice is sex-, age- and genotype-dependent
Due to the epidemic rise of obesity prevalence, adipose tissue (AT) research is of major interest. Our aim was to study specificity of the most-common Cre/loxP approach for inducible gene manipulation of AT in mice (AdipoqCre-ER(T2)). We used mice with tamoxifen-sensitive Cre recombinase controlled...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6959310/ https://www.ncbi.nlm.nih.gov/pubmed/31842670 http://dx.doi.org/10.1080/21623945.2019.1701394 |
Sumario: | Due to the epidemic rise of obesity prevalence, adipose tissue (AT) research is of major interest. Our aim was to study specificity of the most-common Cre/loxP approach for inducible gene manipulation of AT in mice (AdipoqCre-ER(T2)). We used mice with tamoxifen-sensitive Cre recombinase controlled by the adiponectin promoter (AdipoqCre-ER(T2)), which were crossed to a tdTomato reporter mouse to visualize the site of recombination on a single-cell resolution. Albeit tamoxifen induced tdTomato expression in this model, also non-stimulated background recombination (‘Cre leakage’) was detected in AT of untreated Adipoq-CreER(T2)xTDTO mice in vivo. Quantification of Cre leakage revealed age, sex and genotype as factors impacting on non-induced Cre recombination. |
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