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Unspecific DNA recombination in AdipoqCre-ER(T2) – mediated knockout approaches in transgenic mice is sex-, age- and genotype-dependent

Due to the epidemic rise of obesity prevalence, adipose tissue (AT) research is of major interest. Our aim was to study specificity of the most-common Cre/loxP approach for inducible gene manipulation of AT in mice (AdipoqCre-ER(T2)). We used mice with tamoxifen-sensitive Cre recombinase controlled...

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Autores principales: Lindhorst, Andreas, Bechmann, Ingo, Gericke, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6959310/
https://www.ncbi.nlm.nih.gov/pubmed/31842670
http://dx.doi.org/10.1080/21623945.2019.1701394
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author Lindhorst, Andreas
Bechmann, Ingo
Gericke, Martin
author_facet Lindhorst, Andreas
Bechmann, Ingo
Gericke, Martin
author_sort Lindhorst, Andreas
collection PubMed
description Due to the epidemic rise of obesity prevalence, adipose tissue (AT) research is of major interest. Our aim was to study specificity of the most-common Cre/loxP approach for inducible gene manipulation of AT in mice (AdipoqCre-ER(T2)). We used mice with tamoxifen-sensitive Cre recombinase controlled by the adiponectin promoter (AdipoqCre-ER(T2)), which were crossed to a tdTomato reporter mouse to visualize the site of recombination on a single-cell resolution. Albeit tamoxifen induced tdTomato expression in this model, also non-stimulated background recombination (‘Cre leakage’) was detected in AT of untreated Adipoq-CreER(T2)xTDTO mice in vivo. Quantification of Cre leakage revealed age, sex and genotype as factors impacting on non-induced Cre recombination.
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spelling pubmed-69593102020-01-24 Unspecific DNA recombination in AdipoqCre-ER(T2) – mediated knockout approaches in transgenic mice is sex-, age- and genotype-dependent Lindhorst, Andreas Bechmann, Ingo Gericke, Martin Adipocyte Brief Report Due to the epidemic rise of obesity prevalence, adipose tissue (AT) research is of major interest. Our aim was to study specificity of the most-common Cre/loxP approach for inducible gene manipulation of AT in mice (AdipoqCre-ER(T2)). We used mice with tamoxifen-sensitive Cre recombinase controlled by the adiponectin promoter (AdipoqCre-ER(T2)), which were crossed to a tdTomato reporter mouse to visualize the site of recombination on a single-cell resolution. Albeit tamoxifen induced tdTomato expression in this model, also non-stimulated background recombination (‘Cre leakage’) was detected in AT of untreated Adipoq-CreER(T2)xTDTO mice in vivo. Quantification of Cre leakage revealed age, sex and genotype as factors impacting on non-induced Cre recombination. Taylor & Francis 2019-12-16 /pmc/articles/PMC6959310/ /pubmed/31842670 http://dx.doi.org/10.1080/21623945.2019.1701394 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Report
Lindhorst, Andreas
Bechmann, Ingo
Gericke, Martin
Unspecific DNA recombination in AdipoqCre-ER(T2) – mediated knockout approaches in transgenic mice is sex-, age- and genotype-dependent
title Unspecific DNA recombination in AdipoqCre-ER(T2) – mediated knockout approaches in transgenic mice is sex-, age- and genotype-dependent
title_full Unspecific DNA recombination in AdipoqCre-ER(T2) – mediated knockout approaches in transgenic mice is sex-, age- and genotype-dependent
title_fullStr Unspecific DNA recombination in AdipoqCre-ER(T2) – mediated knockout approaches in transgenic mice is sex-, age- and genotype-dependent
title_full_unstemmed Unspecific DNA recombination in AdipoqCre-ER(T2) – mediated knockout approaches in transgenic mice is sex-, age- and genotype-dependent
title_short Unspecific DNA recombination in AdipoqCre-ER(T2) – mediated knockout approaches in transgenic mice is sex-, age- and genotype-dependent
title_sort unspecific dna recombination in adipoqcre-er(t2) – mediated knockout approaches in transgenic mice is sex-, age- and genotype-dependent
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6959310/
https://www.ncbi.nlm.nih.gov/pubmed/31842670
http://dx.doi.org/10.1080/21623945.2019.1701394
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