Site-directed targeting of transcriptional activation-associated proteins to repressed chromatin restores CRISPR activity

Previously, we used an inducible, transgenic polycomb chromatin system to demonstrate that closed, transcriptionally silenced chromatin reduces Cas9 editing. Here, we investigated strategies to enhance Cas9 editing efficiency by artificially perturbing closed chromatin. We tested UNC1999, a small mo...

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Detalles Bibliográficos
Autores principales: Daer, René, Hamna, Fatima, Barrett, Cassandra M., Haynes, Karmella A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2020
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960031/
https://www.ncbi.nlm.nih.gov/pubmed/31967103
http://dx.doi.org/10.1063/1.5127302

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960031/
https://www.ncbi.nlm.nih.gov/pubmed/31967103
http://dx.doi.org/10.1063/1.5127302

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