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Development of protocols for the first serial block-face scanning electron microscopy (SBF SEM) studies of bone tissue

There is an unmet need for a high-resolution three-dimensional (3D) technique to simultaneously image osteocytes and the matrix in which these cells reside. In serial block-face scanning electron microscopy (SBF SEM), an ultramicrotome mounted within the vacuum chamber of a microscope repeatedly sec...

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Detalles Bibliográficos
Autores principales: Goggin, Patricia, Ho, Elaine M.L., Gnaegi, Helmut, Searle, Stuart, Oreffo, Richard O.C., Schneider, Philipp
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961117/
https://www.ncbi.nlm.nih.gov/pubmed/31669251
http://dx.doi.org/10.1016/j.bone.2019.115107