Cargando…

A straightforward approach for bioorthogonal labeling of proteins and organelles in live mammalian cells, using a short peptide tag

BACKGROUND: In the high-resolution microscopy era, genetic code expansion (GCE)-based bioorthogonal labeling offers an elegant way for direct labeling of proteins in live cells with fluorescent dyes. This labeling approach is currently not broadly used in live-cell applications, partly because it ne...

Descripción completa

Detalles Bibliográficos
Autores principales: Segal, Inbar, Nachmias, Dikla, Konig, Andres, Alon, Ariel, Arbely, Eyal, Elia, Natalie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961407/
https://www.ncbi.nlm.nih.gov/pubmed/31937312
http://dx.doi.org/10.1186/s12915-019-0708-7