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A straightforward approach for bioorthogonal labeling of proteins and organelles in live mammalian cells, using a short peptide tag
BACKGROUND: In the high-resolution microscopy era, genetic code expansion (GCE)-based bioorthogonal labeling offers an elegant way for direct labeling of proteins in live cells with fluorescent dyes. This labeling approach is currently not broadly used in live-cell applications, partly because it ne...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961407/ https://www.ncbi.nlm.nih.gov/pubmed/31937312 http://dx.doi.org/10.1186/s12915-019-0708-7 |