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Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum
L-ornithine, a valuable non-protein amino acid, has a wide range of applications in the pharmaceutical and food industries. Currently, microbial fermentation is a promising, sustainable, and environment-friendly method to produce L-ornithine. However, the industrial production capacity of L-ornithin...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6962107/ https://www.ncbi.nlm.nih.gov/pubmed/31998705 http://dx.doi.org/10.3389/fbioe.2019.00440 |
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author | Wu, Xiao-Yu Guo, Xiao-Yan Zhang, Bin Jiang, Yan Ye, Bang-Ce |
author_facet | Wu, Xiao-Yu Guo, Xiao-Yan Zhang, Bin Jiang, Yan Ye, Bang-Ce |
author_sort | Wu, Xiao-Yu |
collection | PubMed |
description | L-ornithine, a valuable non-protein amino acid, has a wide range of applications in the pharmaceutical and food industries. Currently, microbial fermentation is a promising, sustainable, and environment-friendly method to produce L-ornithine. However, the industrial production capacity of L-ornithine by microbial fermentation is low and rarely meets the market demands. Various strategies have been employed to improve the L-ornithine production titers in the model strain, Corynebacterium glutamicum, which serves as a major indicator for improving the cost-effectiveness of L-ornithine production by microbial fermentation. This review focuses on the development of high L-ornithine-producing strains by metabolic engineering and reviews the recent advances in breeding strategies, such as reducing by-product formation, improving the supplementation of precursor glutamate, releasing negative regulation and negative feedback inhibition, increasing the supply of intracellular cofactors, modulating the central metabolic pathway, enhancing the transport system, and adaptive evolution for improving L-ornithine production. |
format | Online Article Text |
id | pubmed-6962107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69621072020-01-29 Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum Wu, Xiao-Yu Guo, Xiao-Yan Zhang, Bin Jiang, Yan Ye, Bang-Ce Front Bioeng Biotechnol Bioengineering and Biotechnology L-ornithine, a valuable non-protein amino acid, has a wide range of applications in the pharmaceutical and food industries. Currently, microbial fermentation is a promising, sustainable, and environment-friendly method to produce L-ornithine. However, the industrial production capacity of L-ornithine by microbial fermentation is low and rarely meets the market demands. Various strategies have been employed to improve the L-ornithine production titers in the model strain, Corynebacterium glutamicum, which serves as a major indicator for improving the cost-effectiveness of L-ornithine production by microbial fermentation. This review focuses on the development of high L-ornithine-producing strains by metabolic engineering and reviews the recent advances in breeding strategies, such as reducing by-product formation, improving the supplementation of precursor glutamate, releasing negative regulation and negative feedback inhibition, increasing the supply of intracellular cofactors, modulating the central metabolic pathway, enhancing the transport system, and adaptive evolution for improving L-ornithine production. Frontiers Media S.A. 2020-01-09 /pmc/articles/PMC6962107/ /pubmed/31998705 http://dx.doi.org/10.3389/fbioe.2019.00440 Text en Copyright © 2020 Wu, Guo, Zhang, Jiang and Ye. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Wu, Xiao-Yu Guo, Xiao-Yan Zhang, Bin Jiang, Yan Ye, Bang-Ce Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum |
title | Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum |
title_full | Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum |
title_fullStr | Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum |
title_full_unstemmed | Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum |
title_short | Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum |
title_sort | recent advances of l-ornithine biosynthesis in metabolically engineered corynebacterium glutamicum |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6962107/ https://www.ncbi.nlm.nih.gov/pubmed/31998705 http://dx.doi.org/10.3389/fbioe.2019.00440 |
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