RNA isoform screens uncover the essentiality and tumor suppressor activity of ultraconserved poison exons

While RNA-seq has enabled comprehensive quantification of alternative splicing, no correspondingly high-throughput assay exists for functionally interrogating individual isoforms. We describe pgFARM (paired guide RNAs for alternative exon removal), a CRISPR/Cas9-based method to manipulate isoforms i...

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Detalles Bibliográficos
Autores principales: Thomas, James D., Polaski, Jacob T., Feng, Qing, De Neef, Emma J., Hoppe, Emma R., McSharry, Maria V., Pangallo, Joseph, Gabel, Austin M., Belleville, Andrea E., Watson, Jacqueline, Nkinsi, Naomi T., Berger, Alice H., Bradley, Robert K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6962552/
https://www.ncbi.nlm.nih.gov/pubmed/31911676
http://dx.doi.org/10.1038/s41588-019-0555-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6962552/
https://www.ncbi.nlm.nih.gov/pubmed/31911676
http://dx.doi.org/10.1038/s41588-019-0555-z

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