Distinguishing Between Monomeric scFv and Diabody in Solution Using Light and Small Angle X-ray Scattering

Depending on the linker length between the V [Formula: see text] and the V [Formula: see text] domain, single-chain Fv (scFv) antibody fragments form monomers, dimers (diabodies) or higher oligomers. We aimed at generating a diabody of the anti-MET antibody 3H3 to use it as crystallization chaperone...

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Detalles Bibliográficos
Autores principales: Lüdel, Frank, Bufe, Sandra, Bleymüller, Willem M., de Jonge, Hugo, Iamele, Luisa, Niemann, Hartmut H., Hellweg, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963988/
https://www.ncbi.nlm.nih.gov/pubmed/31548495
http://dx.doi.org/10.3390/antib8040048

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963988/
https://www.ncbi.nlm.nih.gov/pubmed/31548495
http://dx.doi.org/10.3390/antib8040048

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