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Biodistribution and imaging of an hsp90 ligand labelled with (111)In and (67)Ga for imaging of cell death

BACKGROUND: 4-(N-(S-glutathionylacetyl)amino) phenylarsonous acid (GSAO) when conjugated at the γ-glutamyl residue with fluorophores and radio-isotopes is able to image dead and dying cells in vitro and in vivo by binding to intracellular 90-kDa heat shock proteins (hsp90) when cell membrane integri...

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Autores principales: Ho Shon, Ivan, Kumar, Divesh, Sathiakumar, Chithradevi, Berghofer, Paula, Van, Khang, Chicco, Andrew, Hogg, Philip J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971215/
https://www.ncbi.nlm.nih.gov/pubmed/31960173
http://dx.doi.org/10.1186/s13550-020-0590-x
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author Ho Shon, Ivan
Kumar, Divesh
Sathiakumar, Chithradevi
Berghofer, Paula
Van, Khang
Chicco, Andrew
Hogg, Philip J.
author_facet Ho Shon, Ivan
Kumar, Divesh
Sathiakumar, Chithradevi
Berghofer, Paula
Van, Khang
Chicco, Andrew
Hogg, Philip J.
author_sort Ho Shon, Ivan
collection PubMed
description BACKGROUND: 4-(N-(S-glutathionylacetyl)amino) phenylarsonous acid (GSAO) when conjugated at the γ-glutamyl residue with fluorophores and radio-isotopes is able to image dead and dying cells in vitro and in vivo by binding to intracellular 90-kDa heat shock proteins (hsp90) when cell membrane integrity is compromised. The ability to image cell death has potential clinical impact especially for early treatment response assessment in oncology. This work aims to assess the biodistribution and tumour uptake of diethylene triamine pentaacetic acid GSAO labelled with (111)In ([(111)In]In-DTPA-GSAO) and 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid GSAO labelled with (67)Ga ([(67)Ga]Ga-DOTA-GSAO) in a murine subcutaneous tumour xenograft model and estimate dosimetry of [(67)Ga]Ga-DOTA-GSAO. RESULTS: There was good tumour uptake of both [(111)In]In-DTPA-GSAO and [(67)Ga]Ga-DOTA-GSAO (2.44 ± 0.26% injected activity per gramme of tissue (%IA/g) and 2.75 ± 0.34 %IA/g, respectively) in Balb c nu/nu mice bearing subcutaneous tumour xenografts of a human metastatic prostate cancer cell line (PC3M-luc-c6). Peak tumour uptake occurred at 2.7 h post injection. [(111)In]In-DTPA-GSAO and [(67)Ga]Ga-DOTA-GSAO demonstrated increased uptake in the liver (4.40 ± 0.86 %IA/g and 1.72 ± 0.27 %IA/g, respectively), kidneys (16.54 ± 3.86 %IA/g and 8.16 ± 1.33 %IA/g) and spleen (6.44 ± 1.24 %IA/g and 1.85 ± 0.44 %IA/g); however, uptake in these organs was significantly lower with [(67)Ga]Ga-DOTA-GSAO (p = 0.006, p = 0.017 and p = 0.003, respectively). Uptake of [(67)Ga]Ga-DOTA-GSAO into tumour was higher than all organs except the kidneys. There was negligible uptake in the other organs. Excretion of [(67)Ga]Ga-DOTA-GSAO was more rapid than [(111)In]In-DTPA-GSAO. Estimated effective dose of [(67)Ga]Ga-DOTA-GSAO for an adult male human was 1.54 × 10(− 2) mSv/MBq. CONCLUSIONS: [(67)Ga]Ga-DOTA-GSAO demonstrates higher specific uptake in dead and dying cells within tumours and lower uptake in normal organs than [(111)In]In-DTPA-GSAO. [(67)Ga]Ga-DOTA-GSAO may be potentially useful for imaging cell death in vivo. Dosimetry estimates for [(67)Ga]Ga-DOTA-GSAO are acceptable for future human studies. This work also prepares for development of (68)Ga GSAO radiopharmaceuticals.
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spelling pubmed-69712152020-02-03 Biodistribution and imaging of an hsp90 ligand labelled with (111)In and (67)Ga for imaging of cell death Ho Shon, Ivan Kumar, Divesh Sathiakumar, Chithradevi Berghofer, Paula Van, Khang Chicco, Andrew Hogg, Philip J. EJNMMI Res Original Research BACKGROUND: 4-(N-(S-glutathionylacetyl)amino) phenylarsonous acid (GSAO) when conjugated at the γ-glutamyl residue with fluorophores and radio-isotopes is able to image dead and dying cells in vitro and in vivo by binding to intracellular 90-kDa heat shock proteins (hsp90) when cell membrane integrity is compromised. The ability to image cell death has potential clinical impact especially for early treatment response assessment in oncology. This work aims to assess the biodistribution and tumour uptake of diethylene triamine pentaacetic acid GSAO labelled with (111)In ([(111)In]In-DTPA-GSAO) and 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid GSAO labelled with (67)Ga ([(67)Ga]Ga-DOTA-GSAO) in a murine subcutaneous tumour xenograft model and estimate dosimetry of [(67)Ga]Ga-DOTA-GSAO. RESULTS: There was good tumour uptake of both [(111)In]In-DTPA-GSAO and [(67)Ga]Ga-DOTA-GSAO (2.44 ± 0.26% injected activity per gramme of tissue (%IA/g) and 2.75 ± 0.34 %IA/g, respectively) in Balb c nu/nu mice bearing subcutaneous tumour xenografts of a human metastatic prostate cancer cell line (PC3M-luc-c6). Peak tumour uptake occurred at 2.7 h post injection. [(111)In]In-DTPA-GSAO and [(67)Ga]Ga-DOTA-GSAO demonstrated increased uptake in the liver (4.40 ± 0.86 %IA/g and 1.72 ± 0.27 %IA/g, respectively), kidneys (16.54 ± 3.86 %IA/g and 8.16 ± 1.33 %IA/g) and spleen (6.44 ± 1.24 %IA/g and 1.85 ± 0.44 %IA/g); however, uptake in these organs was significantly lower with [(67)Ga]Ga-DOTA-GSAO (p = 0.006, p = 0.017 and p = 0.003, respectively). Uptake of [(67)Ga]Ga-DOTA-GSAO into tumour was higher than all organs except the kidneys. There was negligible uptake in the other organs. Excretion of [(67)Ga]Ga-DOTA-GSAO was more rapid than [(111)In]In-DTPA-GSAO. Estimated effective dose of [(67)Ga]Ga-DOTA-GSAO for an adult male human was 1.54 × 10(− 2) mSv/MBq. CONCLUSIONS: [(67)Ga]Ga-DOTA-GSAO demonstrates higher specific uptake in dead and dying cells within tumours and lower uptake in normal organs than [(111)In]In-DTPA-GSAO. [(67)Ga]Ga-DOTA-GSAO may be potentially useful for imaging cell death in vivo. Dosimetry estimates for [(67)Ga]Ga-DOTA-GSAO are acceptable for future human studies. This work also prepares for development of (68)Ga GSAO radiopharmaceuticals. Springer Berlin Heidelberg 2020-01-20 /pmc/articles/PMC6971215/ /pubmed/31960173 http://dx.doi.org/10.1186/s13550-020-0590-x Text en © The Author(s). 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Research
Ho Shon, Ivan
Kumar, Divesh
Sathiakumar, Chithradevi
Berghofer, Paula
Van, Khang
Chicco, Andrew
Hogg, Philip J.
Biodistribution and imaging of an hsp90 ligand labelled with (111)In and (67)Ga for imaging of cell death
title Biodistribution and imaging of an hsp90 ligand labelled with (111)In and (67)Ga for imaging of cell death
title_full Biodistribution and imaging of an hsp90 ligand labelled with (111)In and (67)Ga for imaging of cell death
title_fullStr Biodistribution and imaging of an hsp90 ligand labelled with (111)In and (67)Ga for imaging of cell death
title_full_unstemmed Biodistribution and imaging of an hsp90 ligand labelled with (111)In and (67)Ga for imaging of cell death
title_short Biodistribution and imaging of an hsp90 ligand labelled with (111)In and (67)Ga for imaging of cell death
title_sort biodistribution and imaging of an hsp90 ligand labelled with (111)in and (67)ga for imaging of cell death
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971215/
https://www.ncbi.nlm.nih.gov/pubmed/31960173
http://dx.doi.org/10.1186/s13550-020-0590-x
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