A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells

CRISPR-Cas9-mediated gene interference (CRISPRi) and activation (CRISPRa) approaches hold promise for functional gene studies and genome-wide screens in human pluripotent stem cells (hPSCs). However, in contrast to CRISPR-Cas9 nuclease approaches, the efficiency of CRISPRi/a depends on continued exp...

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Detalles Bibliográficos
Autores principales: Hazelbaker, Dane Z., Beccard, Amanda, Angelini, Gabriella, Mazzucato, Patrizia, Messana, Angelica, Lam, Daisy, Eggan, Kevin, Barrett, Lindy E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971260/
https://www.ncbi.nlm.nih.gov/pubmed/31959800
http://dx.doi.org/10.1038/s41598-020-57500-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971260/
https://www.ncbi.nlm.nih.gov/pubmed/31959800
http://dx.doi.org/10.1038/s41598-020-57500-1

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