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Kidney transplant monitoring by urinary flow cytometry: Biomarker combination of T cells, renal tubular epithelial cells, and podocalyxin-positive cells detects rejection

Creatinine and proteinuria are used to monitor kidney transplant patients. However, renal biopsies are needed to diagnose renal graft rejection. Here, we assessed whether the quantification of different urinary cells would allow non-invasive detection of rejection. Urinary cell numbers of CD4(+) and...

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Autores principales: Goerlich, Nina, Brand, Hannah Antonia, Langhans, Valerie, Tesch, Sebastian, Schachtner, Thomas, Koch, Benjamin, Paliege, Alexander, Schneider, Wolfgang, Grützkau, Andreas, Reinke, Petra, Enghard, Philipp
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6972704/
https://www.ncbi.nlm.nih.gov/pubmed/31964937
http://dx.doi.org/10.1038/s41598-020-57524-7
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author Goerlich, Nina
Brand, Hannah Antonia
Langhans, Valerie
Tesch, Sebastian
Schachtner, Thomas
Koch, Benjamin
Paliege, Alexander
Schneider, Wolfgang
Grützkau, Andreas
Reinke, Petra
Enghard, Philipp
author_facet Goerlich, Nina
Brand, Hannah Antonia
Langhans, Valerie
Tesch, Sebastian
Schachtner, Thomas
Koch, Benjamin
Paliege, Alexander
Schneider, Wolfgang
Grützkau, Andreas
Reinke, Petra
Enghard, Philipp
author_sort Goerlich, Nina
collection PubMed
description Creatinine and proteinuria are used to monitor kidney transplant patients. However, renal biopsies are needed to diagnose renal graft rejection. Here, we assessed whether the quantification of different urinary cells would allow non-invasive detection of rejection. Urinary cell numbers of CD4(+) and CD8(+) T cells, monocytes/macrophages, tubular epithelial cells (TEC), and podocalyxin(PDX)-positive cells were determined using flow cytometry and were compared to biopsy results. Urine samples of 63 renal transplant patients were analyzed. Patients with transplant rejection had higher amounts of urinary T cells than controls; however, patients who showed worsening graft function without rejection had similar numbers of T cells. T cells correlated with histological findings (interstitial inflammation p = 0.0005, r = 0.70; tubulitis p = 0.006, r = 0.58). Combining the amount of urinary T cells and TEC, or T cells and PDX(+) cells, yielded a significant segregation of patients with rejection from patients without rejection (all p < 0.01, area under the curve 0.89–0.91). Urinary cell populations analyzed by flow cytometry have the potential to introduce new monitoring methods for kidney transplant patients. The combination of urinary T cells, TEC, and PDX-positive cells may allow non-invasive detection of transplant rejection.
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spelling pubmed-69727042020-01-27 Kidney transplant monitoring by urinary flow cytometry: Biomarker combination of T cells, renal tubular epithelial cells, and podocalyxin-positive cells detects rejection Goerlich, Nina Brand, Hannah Antonia Langhans, Valerie Tesch, Sebastian Schachtner, Thomas Koch, Benjamin Paliege, Alexander Schneider, Wolfgang Grützkau, Andreas Reinke, Petra Enghard, Philipp Sci Rep Article Creatinine and proteinuria are used to monitor kidney transplant patients. However, renal biopsies are needed to diagnose renal graft rejection. Here, we assessed whether the quantification of different urinary cells would allow non-invasive detection of rejection. Urinary cell numbers of CD4(+) and CD8(+) T cells, monocytes/macrophages, tubular epithelial cells (TEC), and podocalyxin(PDX)-positive cells were determined using flow cytometry and were compared to biopsy results. Urine samples of 63 renal transplant patients were analyzed. Patients with transplant rejection had higher amounts of urinary T cells than controls; however, patients who showed worsening graft function without rejection had similar numbers of T cells. T cells correlated with histological findings (interstitial inflammation p = 0.0005, r = 0.70; tubulitis p = 0.006, r = 0.58). Combining the amount of urinary T cells and TEC, or T cells and PDX(+) cells, yielded a significant segregation of patients with rejection from patients without rejection (all p < 0.01, area under the curve 0.89–0.91). Urinary cell populations analyzed by flow cytometry have the potential to introduce new monitoring methods for kidney transplant patients. The combination of urinary T cells, TEC, and PDX-positive cells may allow non-invasive detection of transplant rejection. Nature Publishing Group UK 2020-01-21 /pmc/articles/PMC6972704/ /pubmed/31964937 http://dx.doi.org/10.1038/s41598-020-57524-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Goerlich, Nina
Brand, Hannah Antonia
Langhans, Valerie
Tesch, Sebastian
Schachtner, Thomas
Koch, Benjamin
Paliege, Alexander
Schneider, Wolfgang
Grützkau, Andreas
Reinke, Petra
Enghard, Philipp
Kidney transplant monitoring by urinary flow cytometry: Biomarker combination of T cells, renal tubular epithelial cells, and podocalyxin-positive cells detects rejection
title Kidney transplant monitoring by urinary flow cytometry: Biomarker combination of T cells, renal tubular epithelial cells, and podocalyxin-positive cells detects rejection
title_full Kidney transplant monitoring by urinary flow cytometry: Biomarker combination of T cells, renal tubular epithelial cells, and podocalyxin-positive cells detects rejection
title_fullStr Kidney transplant monitoring by urinary flow cytometry: Biomarker combination of T cells, renal tubular epithelial cells, and podocalyxin-positive cells detects rejection
title_full_unstemmed Kidney transplant monitoring by urinary flow cytometry: Biomarker combination of T cells, renal tubular epithelial cells, and podocalyxin-positive cells detects rejection
title_short Kidney transplant monitoring by urinary flow cytometry: Biomarker combination of T cells, renal tubular epithelial cells, and podocalyxin-positive cells detects rejection
title_sort kidney transplant monitoring by urinary flow cytometry: biomarker combination of t cells, renal tubular epithelial cells, and podocalyxin-positive cells detects rejection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6972704/
https://www.ncbi.nlm.nih.gov/pubmed/31964937
http://dx.doi.org/10.1038/s41598-020-57524-7
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