Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene

BACKGROUND: Delivery of CRISPR reagents into cells as ribonucleoprotein (RNP) complexes enables transient editing, and avoids CRISPR reagent integration in the genomes. Another technical advantage is that RNP delivery can bypass the need of cloning and vector construction steps. In this work we comp...

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Detalles Bibliográficos
Autores principales: Banakar, Raviraj, Schubert, Mollie, Collingwood, Michael, Vakulskas, Christopher, Eggenberger, Alan L., Wang, Kan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6973557/
https://www.ncbi.nlm.nih.gov/pubmed/31965382
http://dx.doi.org/10.1186/s12284-019-0365-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6973557/
https://www.ncbi.nlm.nih.gov/pubmed/31965382
http://dx.doi.org/10.1186/s12284-019-0365-z

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