Cargando…

Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene

BACKGROUND: Delivery of CRISPR reagents into cells as ribonucleoprotein (RNP) complexes enables transient editing, and avoids CRISPR reagent integration in the genomes. Another technical advantage is that RNP delivery can bypass the need of cloning and vector construction steps. In this work we comp...

Descripción completa

Detalles Bibliográficos
Autores principales:	Banakar, Raviraj, Schubert, Mollie, Collingwood, Michael, Vakulskas, Christopher, Eggenberger, Alan L., Wang, Kan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer US 2020
Materias:	Short Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6973557/ https://www.ncbi.nlm.nih.gov/pubmed/31965382 http://dx.doi.org/10.1186/s12284-019-0365-z

Ejemplares similares

Efficiency, Specificity and Temperature Sensitivity of Cas9 and Cas12a RNPs for DNA-free Genome Editing in Plants
por: Banakar, Raviraj, et al.
Publicado: (2022)

Efficient CRISPR/Cas9 Genome Editing of Phytoene desaturase in Cassava
por: Odipio, John, et al.
Publicado: (2017)

Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9
por: Naim, Fatima, et al.
Publicado: (2018)

CRISPR/Cas9-mediated editing of PHYTOENE DESATURASE gene in onion (Allium cepa L.)
por: Mainkar, Pawan, et al.
Publicado: (2023)

CRISPR/Cas9-mediated mutagenesis of phytoene desaturase in diploid and octoploid strawberry
por: Wilson, Fiona M., et al.
Publicado: (2019)

Efficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon
por: Hooghvorst, Isidre, et al.
Publicado: (2019)

CRISPR/Cas9-mediated precise targeted mutagenesis of phytoene desaturase in celery
por: Liu, Jie-Xia, et al.
Publicado: (2022)

Knockout of phytoene desaturase gene using CRISPR/Cas9 in highbush blueberry
por: Vaia, Giuseppe, et al.
Publicado: (2022)

An efficient and specific CRISPR-Cas9 genome editing system targeting soybean phytoene desaturase genes
por: Lu, Qing Shi Mimmie, et al.
Publicado: (2022)

High-frequency random DNA insertions upon co-delivery of CRISPR-Cas9 ribonucleoprotein and selectable marker plasmid in rice
por: Banakar, Raviraj, et al.
Publicado: (2019)

Multiplex CRISPR/Cas9-mediated knockout of the phytoene desaturase gene in Coffea canephora
por: Casarin, Tatiane, et al.
Publicado: (2022)

Silencing of the phytoene desaturase (PDS) gene affects the expression of fruit-ripening genes in tomatoes
por: Naing, Aung Htay, et al.
Publicado: (2019)

Efficient Targeted Mutagenesis Mediated by CRISPR-Cas12a Ribonucleoprotein Complexes in Maize
por: Dong, Shujie, et al.
Publicado: (2021)

Highly Efficient Genome Editing in Plant Protoplasts by Ribonucleoprotein Delivery of CRISPR-Cas12a Nucleases
por: Zhang, Yingxiao, et al.
Publicado: (2022)

Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing
por: Zhang, Song, et al.
Publicado: (2021)

Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
por: Svitashev, Sergei, et al.
Publicado: (2016)

In Vitro Pre-validation of Gene Editing by CRISPR/Cas9 Ribonucleoprotein
por: Mehravar, Maryam, et al.
Publicado: (2019)

Tobacco rattle virus-induced PHYTOENE DESATURASE (PDS) and Mg-chelatase H subunit (ChlH) gene silencing in Solanum pseudocapsicum L.
por: Xu, Hua, et al.
Publicado: (2018)

Establishment of virus-induced gene silencing (VIGS) system in Luffa acutangula using Phytoene desaturase (PDS) and tendril synthesis related gene (TEN)
por: Qi, Xiaoyu, et al.
Publicado: (2023)

Efficient CRISPR/Cas9-Mediated Knockout of an Endogenous PHYTOENE DESATURASE Gene in T1 Progeny of Apomictic Hieracium Enables New Strategies for Apomixis Gene Identification
por: Henderson, Sam W., et al.
Publicado: (2020)

Targeted gene knock-in by CRISPR/Cas ribonucleoproteins in porcine zygotes
por: Park, Ki-Eun, et al.
Publicado: (2017)

Structure of a type IV CRISPR-Cas ribonucleoprotein complex
por: Zhou, Yi, et al.
Publicado: (2021)

CRISPR-Cas9 ribonucleoprotein-mediated co-editing and counterselection in the rice blast fungus
por: Foster, Andrew J., et al.
Publicado: (2018)

CRISPR-Cas9 Ribonucleoprotein-Mediated Genomic Editing in Primary Innate Immune Cells
por: Hildreth, Andrew D., et al.
Publicado: (2020)

Genome editing using preassembled CRISPR-Cas9 ribonucleoprotein complexes in Fusarium graminearum
por: Lee, Nahyun, et al.
Publicado: (2022)

Multiplexed CRISPR gene editing in primary human islet cells with Cas9 ribonucleoprotein
por: Bevacqua, Romina J., et al.
Publicado: (2023)

DNA-Free Genetically Edited Grapevine and Apple Protoplast Using CRISPR/Cas9 Ribonucleoproteins
por: Malnoy, Mickael, et al.
Publicado: (2016)

Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing
por: Chaverra-Rodriguez, Duverney, et al.
Publicado: (2018)

PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts
por: Sant’Ana, Rodrigo Ribeiro Arnt, et al.
Publicado: (2020)

Systemic nanoparticle delivery of CRISPR-Cas9 ribonucleoproteins for effective tissue specific genome editing
por: Wei, Tuo, et al.
Publicado: (2020)

Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method
por: Yu, Lu, et al.
Publicado: (2022)

Genome editing in Penicillium funiculosum using in vitro assembled CRISPR-Cas9 ribonucleoprotein complexes
por: Sinha, Tulika, et al.
Publicado: (2022)

Intragenic Enhancers and Suppressors of Phytoene Desaturase Mutations in Chlamydomonas reinhardtii
por: Tran, Phoi T., et al.
Publicado: (2012)

Plant-type phytoene desaturase: Functional evaluation of structural implications
por: Koschmieder, Julian, et al.
Publicado: (2017)

Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
por: Liang, Zhen, et al.
Publicado: (2017)

Efficient genome editing using CRISPR/Cas9 ribonucleoprotein approach in cultured Medaka fish cells
por: Liu, Qizhi, et al.
Publicado: (2018)

CRISPR/Cas9 Ribonucleoprotein-Based Genome Editing Methodology in the Marine Protozoan Parasite Perkinsus marinus
por: Yadavalli, Raghavendra, et al.
Publicado: (2021)

Dynamics and competition of CRISPR–Cas9 ribonucleoproteins and AAV donor-mediated NHEJ, MMEJ and HDR editing
por: Fu, Ya-Wen, et al.
Publicado: (2021)

Carrier-Free Cellular Transport of CRISPR/Cas9 Ribonucleoprotein for Genome Editing by Cold Atmospheric Plasma
por: Cui, Haodong, et al.
Publicado: (2021)

CRISPR-Cas12a ribonucleoprotein-mediated gene editing in the plant pathogenic fungus Magnaporthe oryzae
por: Huang, Jun, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_36tihm5i6rue895bnd4e5k10ps