Production of genetically engineered mice with higher efficiency, lower mosaicism, and multiplexing capability using maternally expressed Cas9

The CRISPR/Cas9 system is widely used to generate gene-edited animals. Here, we developed an efficient system for generating genetically modified mice using maternal Cas9 from Cas9 transgenic mice. Using this system, we achieved lower mosaicism and higher rates of knock-in success, gene-editing, and...

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Detalles Bibliográficos
Autores principales: Sakurai, Takayuki, Kamiyoshi, Akiko, Kawate, Hisaka, Watanabe, Satoshi, Sato, Masahiro, Shindo, Takayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978307/
https://www.ncbi.nlm.nih.gov/pubmed/31974430
http://dx.doi.org/10.1038/s41598-020-57996-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978307/
https://www.ncbi.nlm.nih.gov/pubmed/31974430
http://dx.doi.org/10.1038/s41598-020-57996-7

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