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Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA

Despite extensive studies on transcription mechanisms, it is unknown how termination complexes are disassembled, especially in what order the essential components dissociate. Our single-molecule fluorescence study unveils that RNA transcript release precedes RNA polymerase (RNAP) dissociation from t...

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Autores principales: Kang, Wooyoung, Ha, Kook Sun, Uhm, Heesoo, Park, Kyuhyong, Lee, Ja Yil, Hohng, Sungchul, Kang, Changwon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978380/
https://www.ncbi.nlm.nih.gov/pubmed/31974350
http://dx.doi.org/10.1038/s41467-019-14200-3
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author Kang, Wooyoung
Ha, Kook Sun
Uhm, Heesoo
Park, Kyuhyong
Lee, Ja Yil
Hohng, Sungchul
Kang, Changwon
author_facet Kang, Wooyoung
Ha, Kook Sun
Uhm, Heesoo
Park, Kyuhyong
Lee, Ja Yil
Hohng, Sungchul
Kang, Changwon
author_sort Kang, Wooyoung
collection PubMed
description Despite extensive studies on transcription mechanisms, it is unknown how termination complexes are disassembled, especially in what order the essential components dissociate. Our single-molecule fluorescence study unveils that RNA transcript release precedes RNA polymerase (RNAP) dissociation from the DNA template much more often than their concurrent dissociations in intrinsic termination of bacterial transcription. As termination is defined by the release of product RNA from the transcription complex, the subsequent retention of RNAP on DNA constitutes a previously unidentified stage, termed here as recycling. During the recycling stage, post-terminational RNAPs one-dimensionally diffuse on DNA in downward and upward directions, and can initiate transcription again at the original and nearby promoters in the case of retaining a sigma factor. The efficiency of this event, termed here as reinitiation, increases with supplement of a sigma factor. In summary, after releasing RNA product at intrinsic termination, recycling RNAP diffuses on the DNA template for reinitiation most of the time.
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spelling pubmed-69783802020-01-27 Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA Kang, Wooyoung Ha, Kook Sun Uhm, Heesoo Park, Kyuhyong Lee, Ja Yil Hohng, Sungchul Kang, Changwon Nat Commun Article Despite extensive studies on transcription mechanisms, it is unknown how termination complexes are disassembled, especially in what order the essential components dissociate. Our single-molecule fluorescence study unveils that RNA transcript release precedes RNA polymerase (RNAP) dissociation from the DNA template much more often than their concurrent dissociations in intrinsic termination of bacterial transcription. As termination is defined by the release of product RNA from the transcription complex, the subsequent retention of RNAP on DNA constitutes a previously unidentified stage, termed here as recycling. During the recycling stage, post-terminational RNAPs one-dimensionally diffuse on DNA in downward and upward directions, and can initiate transcription again at the original and nearby promoters in the case of retaining a sigma factor. The efficiency of this event, termed here as reinitiation, increases with supplement of a sigma factor. In summary, after releasing RNA product at intrinsic termination, recycling RNAP diffuses on the DNA template for reinitiation most of the time. Nature Publishing Group UK 2020-01-23 /pmc/articles/PMC6978380/ /pubmed/31974350 http://dx.doi.org/10.1038/s41467-019-14200-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kang, Wooyoung
Ha, Kook Sun
Uhm, Heesoo
Park, Kyuhyong
Lee, Ja Yil
Hohng, Sungchul
Kang, Changwon
Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA
title Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA
title_full Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA
title_fullStr Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA
title_full_unstemmed Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA
title_short Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA
title_sort transcription reinitiation by recycling rna polymerase that diffuses on dna after releasing terminated rna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978380/
https://www.ncbi.nlm.nih.gov/pubmed/31974350
http://dx.doi.org/10.1038/s41467-019-14200-3
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