Quantitative Intracellular pH Determinations in Single Live Mammalian Spermatozoa Using the Ratiometric Dye SNARF-5F

Intracellular pH (pH(i)) plays a crucial role in mammalian sperm physiology. However, it is a challenging task to acquire quantitative single sperm pH(i) images due to their small size and beating flagella. In this study, we established a robust pH(i) imaging system using the dual-emission ratiometr...

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Detalles Bibliográficos
Autores principales: Chávez, Julio C., Darszon, Alberto, Treviño, Claudia L., Nishigaki, Takuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Cell and Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978660/
https://www.ncbi.nlm.nih.gov/pubmed/32010689
http://dx.doi.org/10.3389/fcell.2019.00366

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978660/
https://www.ncbi.nlm.nih.gov/pubmed/32010689
http://dx.doi.org/10.3389/fcell.2019.00366

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