Cargando…
Quantitative Intracellular pH Determinations in Single Live Mammalian Spermatozoa Using the Ratiometric Dye SNARF-5F
Intracellular pH (pH(i)) plays a crucial role in mammalian sperm physiology. However, it is a challenging task to acquire quantitative single sperm pH(i) images due to their small size and beating flagella. In this study, we established a robust pH(i) imaging system using the dual-emission ratiometr...
| Autores principales: | , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2020
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978660/ https://www.ncbi.nlm.nih.gov/pubmed/32010689 http://dx.doi.org/10.3389/fcell.2019.00366 |