Nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins

Multi-pass membrane proteins are important targets of biologic medicines. Given the inherent difficulties in working with membrane proteins, we sought to investigate the utility of membrane scaffold protein nanodiscs as a means of solubilizing membrane proteins to aid antibody discovery. Using a mod...

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Autores principales: Gardill, Bernd, Huang, Jerry, Tu, Lawrence, Van Petegem, Filip, Oxenoid, Kirill, Thomson, Christy A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981118/
https://www.ncbi.nlm.nih.gov/pubmed/31980674
http://dx.doi.org/10.1038/s41598-020-58002-w
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author Gardill, Bernd
Huang, Jerry
Tu, Lawrence
Van Petegem, Filip
Oxenoid, Kirill
Thomson, Christy A.
author_facet Gardill, Bernd
Huang, Jerry
Tu, Lawrence
Van Petegem, Filip
Oxenoid, Kirill
Thomson, Christy A.
author_sort Gardill, Bernd
collection PubMed
description Multi-pass membrane proteins are important targets of biologic medicines. Given the inherent difficulties in working with membrane proteins, we sought to investigate the utility of membrane scaffold protein nanodiscs as a means of solubilizing membrane proteins to aid antibody discovery. Using a model multi-pass membrane protein, we demonstrate how incorporation of a multi-pass membrane protein into nanodiscs can be used in flow cytometry to identify antigen-specific hybridoma. The use of target protein-loaded nanodiscs to sort individual hybridoma early in the screening process can reduce the time required to identify antibodies against multi-pass membrane proteins.
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spelling pubmed-69811182020-01-30 Nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins Gardill, Bernd Huang, Jerry Tu, Lawrence Van Petegem, Filip Oxenoid, Kirill Thomson, Christy A. Sci Rep Article Multi-pass membrane proteins are important targets of biologic medicines. Given the inherent difficulties in working with membrane proteins, we sought to investigate the utility of membrane scaffold protein nanodiscs as a means of solubilizing membrane proteins to aid antibody discovery. Using a model multi-pass membrane protein, we demonstrate how incorporation of a multi-pass membrane protein into nanodiscs can be used in flow cytometry to identify antigen-specific hybridoma. The use of target protein-loaded nanodiscs to sort individual hybridoma early in the screening process can reduce the time required to identify antibodies against multi-pass membrane proteins. Nature Publishing Group UK 2020-01-24 /pmc/articles/PMC6981118/ /pubmed/31980674 http://dx.doi.org/10.1038/s41598-020-58002-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Gardill, Bernd
Huang, Jerry
Tu, Lawrence
Van Petegem, Filip
Oxenoid, Kirill
Thomson, Christy A.
Nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins
title Nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins
title_full Nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins
title_fullStr Nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins
title_full_unstemmed Nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins
title_short Nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins
title_sort nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981118/
https://www.ncbi.nlm.nih.gov/pubmed/31980674
http://dx.doi.org/10.1038/s41598-020-58002-w
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