Cargando…

Chitosan Application in Vineyards (Vitis vinifera L. cv. Tinto Cão) Induces Accumulation of Anthocyanins and Other Phenolics in Berries, Mediated by Modifications in the Transcription of Secondary Metabolism Genes

Despite the numerous beneficial properties and uses of chitosan in agriculture, the molecular mechanisms behind its elicitation potential are still unclear. This study aimed at understanding the effect of chitosan application in the levels of phenolic compounds of Vitis vinifera L. red grapes berry...

Descripción completa

Detalles Bibliográficos
Autores principales: Singh, Rupesh Kumar, Martins, Viviana, Soares, Bruno, Castro, Isaura, Falco, Virgílio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981802/
https://www.ncbi.nlm.nih.gov/pubmed/31906425
http://dx.doi.org/10.3390/ijms21010306
_version_ 1783491166171299840
author Singh, Rupesh Kumar
Martins, Viviana
Soares, Bruno
Castro, Isaura
Falco, Virgílio
author_facet Singh, Rupesh Kumar
Martins, Viviana
Soares, Bruno
Castro, Isaura
Falco, Virgílio
author_sort Singh, Rupesh Kumar
collection PubMed
description Despite the numerous beneficial properties and uses of chitosan in agriculture, the molecular mechanisms behind its elicitation potential are still unclear. This study aimed at understanding the effect of chitosan application in the levels of phenolic compounds of Vitis vinifera L. red grapes berry skin (cv. Tinto Cão) during veraison. Grapevines were treated with chitosan (0.01% in 0.01% acetic acid) while control grapevines were sprayed with 0.01% acetic acid. Results showed that several monomeric anthocyanins increased significantly in berry skins after treatment with chitosan. Additionally, Catechin, Rutin and Querecetin-3-O-galactoside were also recorded in higher amount upon chitosan treatment. Besides modulating the phenolic content, chitosan treatment also induced modifications in several target genes encoding key enzymes and transporters involved in secondary metabolic pathways. For instance, the genes PAL, CHS, F3H, ANR, UFGT, ABCC1, GST, MATE1 were upregulated in leaves and berry skins at veraison cessation in response to chitosan treatment. Overall, the results demonstrated that chitosan has a stimulatory effect on the accumulation of phenolic compounds, including anthocyanins, mediated by modifications in the transcription of key genes involved in their biosynthesis and transport in grape berries.
format Online
Article
Text
id pubmed-6981802
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69818022020-02-07 Chitosan Application in Vineyards (Vitis vinifera L. cv. Tinto Cão) Induces Accumulation of Anthocyanins and Other Phenolics in Berries, Mediated by Modifications in the Transcription of Secondary Metabolism Genes Singh, Rupesh Kumar Martins, Viviana Soares, Bruno Castro, Isaura Falco, Virgílio Int J Mol Sci Communication Despite the numerous beneficial properties and uses of chitosan in agriculture, the molecular mechanisms behind its elicitation potential are still unclear. This study aimed at understanding the effect of chitosan application in the levels of phenolic compounds of Vitis vinifera L. red grapes berry skin (cv. Tinto Cão) during veraison. Grapevines were treated with chitosan (0.01% in 0.01% acetic acid) while control grapevines were sprayed with 0.01% acetic acid. Results showed that several monomeric anthocyanins increased significantly in berry skins after treatment with chitosan. Additionally, Catechin, Rutin and Querecetin-3-O-galactoside were also recorded in higher amount upon chitosan treatment. Besides modulating the phenolic content, chitosan treatment also induced modifications in several target genes encoding key enzymes and transporters involved in secondary metabolic pathways. For instance, the genes PAL, CHS, F3H, ANR, UFGT, ABCC1, GST, MATE1 were upregulated in leaves and berry skins at veraison cessation in response to chitosan treatment. Overall, the results demonstrated that chitosan has a stimulatory effect on the accumulation of phenolic compounds, including anthocyanins, mediated by modifications in the transcription of key genes involved in their biosynthesis and transport in grape berries. MDPI 2020-01-02 /pmc/articles/PMC6981802/ /pubmed/31906425 http://dx.doi.org/10.3390/ijms21010306 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Singh, Rupesh Kumar
Martins, Viviana
Soares, Bruno
Castro, Isaura
Falco, Virgílio
Chitosan Application in Vineyards (Vitis vinifera L. cv. Tinto Cão) Induces Accumulation of Anthocyanins and Other Phenolics in Berries, Mediated by Modifications in the Transcription of Secondary Metabolism Genes
title Chitosan Application in Vineyards (Vitis vinifera L. cv. Tinto Cão) Induces Accumulation of Anthocyanins and Other Phenolics in Berries, Mediated by Modifications in the Transcription of Secondary Metabolism Genes
title_full Chitosan Application in Vineyards (Vitis vinifera L. cv. Tinto Cão) Induces Accumulation of Anthocyanins and Other Phenolics in Berries, Mediated by Modifications in the Transcription of Secondary Metabolism Genes
title_fullStr Chitosan Application in Vineyards (Vitis vinifera L. cv. Tinto Cão) Induces Accumulation of Anthocyanins and Other Phenolics in Berries, Mediated by Modifications in the Transcription of Secondary Metabolism Genes
title_full_unstemmed Chitosan Application in Vineyards (Vitis vinifera L. cv. Tinto Cão) Induces Accumulation of Anthocyanins and Other Phenolics in Berries, Mediated by Modifications in the Transcription of Secondary Metabolism Genes
title_short Chitosan Application in Vineyards (Vitis vinifera L. cv. Tinto Cão) Induces Accumulation of Anthocyanins and Other Phenolics in Berries, Mediated by Modifications in the Transcription of Secondary Metabolism Genes
title_sort chitosan application in vineyards (vitis vinifera l. cv. tinto cão) induces accumulation of anthocyanins and other phenolics in berries, mediated by modifications in the transcription of secondary metabolism genes
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6981802/
https://www.ncbi.nlm.nih.gov/pubmed/31906425
http://dx.doi.org/10.3390/ijms21010306
work_keys_str_mv AT singhrupeshkumar chitosanapplicationinvineyardsvitisviniferalcvtintocaoinducesaccumulationofanthocyaninsandotherphenolicsinberriesmediatedbymodificationsinthetranscriptionofsecondarymetabolismgenes
AT martinsviviana chitosanapplicationinvineyardsvitisviniferalcvtintocaoinducesaccumulationofanthocyaninsandotherphenolicsinberriesmediatedbymodificationsinthetranscriptionofsecondarymetabolismgenes
AT soaresbruno chitosanapplicationinvineyardsvitisviniferalcvtintocaoinducesaccumulationofanthocyaninsandotherphenolicsinberriesmediatedbymodificationsinthetranscriptionofsecondarymetabolismgenes
AT castroisaura chitosanapplicationinvineyardsvitisviniferalcvtintocaoinducesaccumulationofanthocyaninsandotherphenolicsinberriesmediatedbymodificationsinthetranscriptionofsecondarymetabolismgenes
AT falcovirgilio chitosanapplicationinvineyardsvitisviniferalcvtintocaoinducesaccumulationofanthocyaninsandotherphenolicsinberriesmediatedbymodificationsinthetranscriptionofsecondarymetabolismgenes