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Targeting the 16S rRNA Gene for Bacterial Identification in Complex Mixed Samples: Comparative Evaluation of Second (Illumina) and Third (Oxford Nanopore Technologies) Generation Sequencing Technologies

Rapid, accurate bacterial identification in biological samples is an important task for microbiology laboratories, for which 16S rRNA gene Sanger sequencing of cultured isolates is frequently used. In contrast, next-generation sequencing does not require intermediate culturing steps and can be direc...

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Detalles Bibliográficos
Autores principales: Winand, Raf, Bogaerts, Bert, Hoffman, Stefan, Lefevre, Loïc, Delvoye, Maud, Van Braekel, Julien, Fu, Qiang, Roosens, Nancy HC, De Keersmaecker, Sigrid CJ, Vanneste, Kevin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982111/
https://www.ncbi.nlm.nih.gov/pubmed/31906254
http://dx.doi.org/10.3390/ijms21010298