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Immobilization of Allantoinase for the Development of an Optical Biosensor of Oxidative Stress States

Allantoin, the natural end product of purine catabolism in mammals, is non-enzymatically produced from the scavenging of reactive oxygen species through the degradation of uric acid. Levels of allantoin in biological fluids are sensitively influenced by the presence of free radicals, making this mol...

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Autores principales: Marchetti, Marialaura, Ronda, Luca, Percudani, Riccardo, Bettati, Stefano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983136/
https://www.ncbi.nlm.nih.gov/pubmed/31905788
http://dx.doi.org/10.3390/s20010196
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author Marchetti, Marialaura
Ronda, Luca
Percudani, Riccardo
Bettati, Stefano
author_facet Marchetti, Marialaura
Ronda, Luca
Percudani, Riccardo
Bettati, Stefano
author_sort Marchetti, Marialaura
collection PubMed
description Allantoin, the natural end product of purine catabolism in mammals, is non-enzymatically produced from the scavenging of reactive oxygen species through the degradation of uric acid. Levels of allantoin in biological fluids are sensitively influenced by the presence of free radicals, making this molecule a candidate marker of acute oxidative stress in clinical analyses. With this aim, we exploited allantoinase—the enzyme responsible for allantoin hydrolization in plants and lower organisms—for the development of a biosensor exploiting a fast enzymatic-chemical assay for allantoin quantification. Recombinant allantoinase was entrapped in a wet nanoporous silica gel matrix and its structural properties, function, and stability were characterized through fluorescence spectroscopy and circular dichroism measurements, and compared to the soluble enzyme. Physical immobilization in silica gel minimally influences the structure and the catalytic efficiency of entrapped allantoinase, which can be reused several times and stored for several months with good activity retention. These results, together with the relative ease of the sol-gel preparation and handling, make the encapsulated allantoinase a good candidate for the development of an allantoin biosensor.
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spelling pubmed-69831362020-02-06 Immobilization of Allantoinase for the Development of an Optical Biosensor of Oxidative Stress States Marchetti, Marialaura Ronda, Luca Percudani, Riccardo Bettati, Stefano Sensors (Basel) Article Allantoin, the natural end product of purine catabolism in mammals, is non-enzymatically produced from the scavenging of reactive oxygen species through the degradation of uric acid. Levels of allantoin in biological fluids are sensitively influenced by the presence of free radicals, making this molecule a candidate marker of acute oxidative stress in clinical analyses. With this aim, we exploited allantoinase—the enzyme responsible for allantoin hydrolization in plants and lower organisms—for the development of a biosensor exploiting a fast enzymatic-chemical assay for allantoin quantification. Recombinant allantoinase was entrapped in a wet nanoporous silica gel matrix and its structural properties, function, and stability were characterized through fluorescence spectroscopy and circular dichroism measurements, and compared to the soluble enzyme. Physical immobilization in silica gel minimally influences the structure and the catalytic efficiency of entrapped allantoinase, which can be reused several times and stored for several months with good activity retention. These results, together with the relative ease of the sol-gel preparation and handling, make the encapsulated allantoinase a good candidate for the development of an allantoin biosensor. MDPI 2019-12-29 /pmc/articles/PMC6983136/ /pubmed/31905788 http://dx.doi.org/10.3390/s20010196 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Marchetti, Marialaura
Ronda, Luca
Percudani, Riccardo
Bettati, Stefano
Immobilization of Allantoinase for the Development of an Optical Biosensor of Oxidative Stress States
title Immobilization of Allantoinase for the Development of an Optical Biosensor of Oxidative Stress States
title_full Immobilization of Allantoinase for the Development of an Optical Biosensor of Oxidative Stress States
title_fullStr Immobilization of Allantoinase for the Development of an Optical Biosensor of Oxidative Stress States
title_full_unstemmed Immobilization of Allantoinase for the Development of an Optical Biosensor of Oxidative Stress States
title_short Immobilization of Allantoinase for the Development of an Optical Biosensor of Oxidative Stress States
title_sort immobilization of allantoinase for the development of an optical biosensor of oxidative stress states
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6983136/
https://www.ncbi.nlm.nih.gov/pubmed/31905788
http://dx.doi.org/10.3390/s20010196
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