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Decrease of Cardiac Parkin Protein in Obese Mice

Mitophagy plays a major role in heart physiology. Impairment of Parkin-dependent mitophagy in heart is known to be deleterious. Obesity is a known cardiovascular risk factor. Impaired autophagy has been reported in models of obesity or hyperlipidemia/hypercholesterolemia; however less is known regar...

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Autores principales: Thomas, Amandine, Marek-Iannucci, Stefanie, Tucker, Kyle C., Andres, Allen M., Gottlieb, Roberta A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6984192/
https://www.ncbi.nlm.nih.gov/pubmed/32039238
http://dx.doi.org/10.3389/fcvm.2019.00191
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author Thomas, Amandine
Marek-Iannucci, Stefanie
Tucker, Kyle C.
Andres, Allen M.
Gottlieb, Roberta A.
author_facet Thomas, Amandine
Marek-Iannucci, Stefanie
Tucker, Kyle C.
Andres, Allen M.
Gottlieb, Roberta A.
author_sort Thomas, Amandine
collection PubMed
description Mitophagy plays a major role in heart physiology. Impairment of Parkin-dependent mitophagy in heart is known to be deleterious. Obesity is a known cardiovascular risk factor. Impaired autophagy has been reported in models of obesity or hyperlipidemia/hypercholesterolemia; however less is known regarding obesity and mitophagy. The aim of this study was to evaluate the regulation of Parkin expression in hearts of mice fed a high fat diet. Interestingly, we found a significant decrease in Parkin protein in hearts of HFD mice compared those fed a low-fat diet. This was associated with mitochondrial dysfunction in the context of ischemia/reperfusion (I/R). This downregulation was not associated with a decrease in Parkin mRNA expression. We did not detect any change in the degradation rate of Parkin and only a slight decrease in its translation. The reduction of Parkin protein abundance in HFD hearts remains a mystery and will need further studies. However, Parkin depletion in the setting of obesity may contribute to cardiovascular risk.
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spelling pubmed-69841922020-02-07 Decrease of Cardiac Parkin Protein in Obese Mice Thomas, Amandine Marek-Iannucci, Stefanie Tucker, Kyle C. Andres, Allen M. Gottlieb, Roberta A. Front Cardiovasc Med Cardiovascular Medicine Mitophagy plays a major role in heart physiology. Impairment of Parkin-dependent mitophagy in heart is known to be deleterious. Obesity is a known cardiovascular risk factor. Impaired autophagy has been reported in models of obesity or hyperlipidemia/hypercholesterolemia; however less is known regarding obesity and mitophagy. The aim of this study was to evaluate the regulation of Parkin expression in hearts of mice fed a high fat diet. Interestingly, we found a significant decrease in Parkin protein in hearts of HFD mice compared those fed a low-fat diet. This was associated with mitochondrial dysfunction in the context of ischemia/reperfusion (I/R). This downregulation was not associated with a decrease in Parkin mRNA expression. We did not detect any change in the degradation rate of Parkin and only a slight decrease in its translation. The reduction of Parkin protein abundance in HFD hearts remains a mystery and will need further studies. However, Parkin depletion in the setting of obesity may contribute to cardiovascular risk. Frontiers Media S.A. 2020-01-20 /pmc/articles/PMC6984192/ /pubmed/32039238 http://dx.doi.org/10.3389/fcvm.2019.00191 Text en Copyright © 2020 Thomas, Marek-Iannucci, Tucker, Andres and Gottlieb. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Thomas, Amandine
Marek-Iannucci, Stefanie
Tucker, Kyle C.
Andres, Allen M.
Gottlieb, Roberta A.
Decrease of Cardiac Parkin Protein in Obese Mice
title Decrease of Cardiac Parkin Protein in Obese Mice
title_full Decrease of Cardiac Parkin Protein in Obese Mice
title_fullStr Decrease of Cardiac Parkin Protein in Obese Mice
title_full_unstemmed Decrease of Cardiac Parkin Protein in Obese Mice
title_short Decrease of Cardiac Parkin Protein in Obese Mice
title_sort decrease of cardiac parkin protein in obese mice
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6984192/
https://www.ncbi.nlm.nih.gov/pubmed/32039238
http://dx.doi.org/10.3389/fcvm.2019.00191
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